Abnormal Ca2+-calmodulin-dependent protein kinase II function mediates synaptic and motor deficits in experimental parkinsonism

Barbara Picconi, Fabrizio Gardoni, Diego Centonze, Daniela Mauceri, M. Angela Cenci, Giorgio Bernardi, Paolo Calabresi, Monica Di Luca

Research output: Contribution to journalArticle

Abstract

The NMDA receptor complex represents a key molecular element in the pathogenesis of long-term synaptic changes and motor abnormalities in Parkinson's disease (PD). Here we show that NMDA receptor 1 (NR1) subunit and postsynaptic density (PSD)-95 protein levels are selectively reduced in the PSD of dopamine (DA)-denervated striata. These effects are accompanied by an increase in striatal levels of αCa2+-calmodulin-dependent protein kinase II (αCaMKII) autophosphorylation, along with a higher recruitment of activated αCaMKII to the regulatory NMDA receptor NR2A-NR2B subunits. Acute treatment of striatal slices with R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine hydrochloride, but not with L-sulpiride, mimicked the effect of DA denervation on both αCaMKII autophosphorylation and corticostriatal synaptic plasticity. In addition to normalizing αCaMKII autophosphorylation levels as well as assembly and anchoring of the kinase to the NMDA receptor complex, intrastriatal administration of the CaMKII inhibitors KN-93 (N-[2-[[[3-(4-chlorophenyl)-2-propenyl]methylamino]methyl]phenyl] -N-(2-hydroxyethyl)-4-methoxybenzenesulfonamide) and antennapedia autocamtide-related inhibitory peptide II is able to reverse both the alterations in corticostriatal synaptic plasticity and the deficits in spontaneous motor behavior that are found in an animal model of PD. The same beneficial effects are produced by a regimen of L-3,4-dihydroxyphenylalanine (L-DOPA) treatment, which is able to normalize αCaMKII autophosphorylation. These data indicate that abnormal αCaMKII autophosphorylation plays a causal role in the alterations of striatal plasticity and motor behavior that follow DA denervation. Normalization of CaMKII activity may be an important underlying mechanism of the therapeutic action of L-DOPA in PD.

Original languageEnglish
Pages (from-to)5283-5291
Number of pages9
JournalJournal of Neuroscience
Volume24
Issue number23
DOIs
Publication statusPublished - Jun 9 2004

Keywords

  • 6-OHDA
  • CaMKII
  • LTP
  • Parkinson's disease
  • Rat
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Abnormal Ca2+-calmodulin-dependent protein kinase II function mediates synaptic and motor deficits in experimental parkinsonism'. Together they form a unique fingerprint.

  • Cite this