TY - JOUR
T1 - Abnormal cytology in oropharyngeal brushings and in oral rinses is not associated with HPV infection
T2 - The OHMAR study
AU - Benevolo, Maria
AU - Rollo, Francesca
AU - Giuliani, Massimo
AU - Pichi, Barbara
AU - Latini, Alessandra
AU - Pellini, Raul
AU - Vescio, Maria Fenicia
AU - Morrone, Aldo
AU - Cristaudo, Antonio
AU - Donà, Maria Gabriella
N1 - Funding Information:
This work was supported by the Italian Ministry of Health (GR-2011-02349732 to Maria Gabriella Don?).
Publisher Copyright:
© 2020 American Cancer Society
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Background: No screening is available for human papillomavirus (HPV)-associated oropharyngeal cancers. The authors investigated whether cytology may be used as a screening tool and whether oral HPV infection is associated with cytologic abnormalities detected in oropharyngeal brushings and in oral rinse-and-gargle specimens from asymptomatic individuals at increased risk for oral HPV infection. Methods: Specimens were collected from men who have sex with men at 6-month intervals. Oropharyngeal samples and oral rinse-and-gargle specimens were collected using a cytobrush and mouthwash, respectively. Exfoliated cells were dispersed in PreservCyt. Liquid-based slides were stained with Papanicolaou. An HPV genotyping test using a linear array was used for HPV detection. Associations with abnormal cytology were investigated using logistic regression. Results: Overall, 631 brushings and 802 rinses collected from 310 individuals were evaluated; of these specimens, 2 brushings (0.3%) and 10 rinses (1.2%) were inadequate for morphologic evaluation. Of the adequate samples, 35 of 629 brushings (5.5%) and 19 of 792 rinses (2.4%) were abnormal. No associations of high-risk HPVs or HPV-16 infection with cytologic abnormalities were observed for oropharyngeal brushings (high-risk HPVs: odds ratio [OR], 1.19; 95% CI, 0.41-3.50; P =.75; HPV-16: OR, 0.76; 95% CI, 0.10-5.84; P =.79) or for oral rinses (high-risk HPVs: OR, 1.13; 95% CI, 0.26-4.98; P =.87; HPV-16: OR, 0.62; 95% CI, 0.04-10.60; P =.74). Concurrent moderate/heavy drinking and smoking significantly increased the risk of cytologic abnormalities in the brushings (hazard ratio, 4.84; 95% CI, 1.15-20.43; P =.03). Conclusions: Oral HPV infection by high-risk HPVs and HPV-16 does not confer an increased risk of cytologic abnormalities in oropharyngeal brushings and oral rinses. Abnormal cytology seems to be associated with smoking and drinking habits.
AB - Background: No screening is available for human papillomavirus (HPV)-associated oropharyngeal cancers. The authors investigated whether cytology may be used as a screening tool and whether oral HPV infection is associated with cytologic abnormalities detected in oropharyngeal brushings and in oral rinse-and-gargle specimens from asymptomatic individuals at increased risk for oral HPV infection. Methods: Specimens were collected from men who have sex with men at 6-month intervals. Oropharyngeal samples and oral rinse-and-gargle specimens were collected using a cytobrush and mouthwash, respectively. Exfoliated cells were dispersed in PreservCyt. Liquid-based slides were stained with Papanicolaou. An HPV genotyping test using a linear array was used for HPV detection. Associations with abnormal cytology were investigated using logistic regression. Results: Overall, 631 brushings and 802 rinses collected from 310 individuals were evaluated; of these specimens, 2 brushings (0.3%) and 10 rinses (1.2%) were inadequate for morphologic evaluation. Of the adequate samples, 35 of 629 brushings (5.5%) and 19 of 792 rinses (2.4%) were abnormal. No associations of high-risk HPVs or HPV-16 infection with cytologic abnormalities were observed for oropharyngeal brushings (high-risk HPVs: odds ratio [OR], 1.19; 95% CI, 0.41-3.50; P =.75; HPV-16: OR, 0.76; 95% CI, 0.10-5.84; P =.79) or for oral rinses (high-risk HPVs: OR, 1.13; 95% CI, 0.26-4.98; P =.87; HPV-16: OR, 0.62; 95% CI, 0.04-10.60; P =.74). Concurrent moderate/heavy drinking and smoking significantly increased the risk of cytologic abnormalities in the brushings (hazard ratio, 4.84; 95% CI, 1.15-20.43; P =.03). Conclusions: Oral HPV infection by high-risk HPVs and HPV-16 does not confer an increased risk of cytologic abnormalities in oropharyngeal brushings and oral rinses. Abnormal cytology seems to be associated with smoking and drinking habits.
KW - brushing
KW - HIV
KW - human papillomavirus (HPV)
KW - liquid-based cytology
KW - men who have sex with men (MSM)
KW - oral rinse
KW - oropharyngeal neoplasms
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UR - http://www.scopus.com/inward/citedby.url?scp=85085709775&partnerID=8YFLogxK
U2 - 10.1002/cncy.22295
DO - 10.1002/cncy.22295
M3 - Article
C2 - 32485093
AN - SCOPUS:85085709775
VL - 128
SP - 648
EP - 655
JO - Cancer cytopathology
JF - Cancer cytopathology
SN - 1934-662X
IS - 9
ER -