Abnormal expression of neuronal nitric oxide synthase triggers limbic seizures and hippocampal damage in rat

Giacinto Bagetta, Anna Maria Paoletti, Aida Leta, Claudio Del Duca, Robert Nisticò, D. Rotiroti, M. Tiziana Corasaniti

Research output: Contribution to journalArticle

Abstract

Administration of tacrine (5 mg/kg ip), an anticho-linesterase agent, in rats pretreated (24 h beforehand) with lithium chloride (LiCl; 12 mEq/kg ip) provides a useful experimental model to study limbic seizures and delayed hippocampal damage. Here we report Western blotting evidence demonstrating that in rat LiCl and tacrine enhance the expression of neuronal nitric oxide synthase (nNOS), but not eNOS, enzyme protein in the hippocampus during the preconvulsive period and this triggers seizures and hippocampal damage. In fact, systemic administration of 7-nitro indazole (7-NI; 50 mg/kg given ip 30 min before tacrine), a selective inhibitor of nNOS, prevented the expression of motor and electrocortical (ECoG) seizures and abolished neuronal cell death in the hippocampus. A lower dose (5 mg/kg ip) of 7-NI was ineffective. In conclusion, the present data support a role for abnormal nNOS expression in the mechanism which triggers limbic seizures and delayed excitotoxic damage in the hippocampus of rat.

Original languageEnglish
Pages (from-to)255-260
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume291
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

Tacrine
Nitric Oxide Synthase Type I
Rats
Seizures
Hippocampus
Lithium Chloride
Cell death
Cell Death
Theoretical Models
Western Blotting
Enzymes
Proteins

Keywords

  • 7-nitro indazole
  • ENOS
  • LiCl
  • Neuronal death
  • NNOS
  • NO
  • Seizures
  • Tacrine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Abnormal expression of neuronal nitric oxide synthase triggers limbic seizures and hippocampal damage in rat. / Bagetta, Giacinto; Paoletti, Anna Maria; Leta, Aida; Del Duca, Claudio; Nisticò, Robert; Rotiroti, D.; Corasaniti, M. Tiziana.

In: Biochemical and Biophysical Research Communications, Vol. 291, No. 2, 2002, p. 255-260.

Research output: Contribution to journalArticle

Bagetta, G, Paoletti, AM, Leta, A, Del Duca, C, Nisticò, R, Rotiroti, D & Corasaniti, MT 2002, 'Abnormal expression of neuronal nitric oxide synthase triggers limbic seizures and hippocampal damage in rat', Biochemical and Biophysical Research Communications, vol. 291, no. 2, pp. 255-260. https://doi.org/10.1006/bbrc.2002.6424
Bagetta, Giacinto ; Paoletti, Anna Maria ; Leta, Aida ; Del Duca, Claudio ; Nisticò, Robert ; Rotiroti, D. ; Corasaniti, M. Tiziana. / Abnormal expression of neuronal nitric oxide synthase triggers limbic seizures and hippocampal damage in rat. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 291, No. 2. pp. 255-260.
@article{e5cacaccf70a4127aedfdfb1e865c16a,
title = "Abnormal expression of neuronal nitric oxide synthase triggers limbic seizures and hippocampal damage in rat",
abstract = "Administration of tacrine (5 mg/kg ip), an anticho-linesterase agent, in rats pretreated (24 h beforehand) with lithium chloride (LiCl; 12 mEq/kg ip) provides a useful experimental model to study limbic seizures and delayed hippocampal damage. Here we report Western blotting evidence demonstrating that in rat LiCl and tacrine enhance the expression of neuronal nitric oxide synthase (nNOS), but not eNOS, enzyme protein in the hippocampus during the preconvulsive period and this triggers seizures and hippocampal damage. In fact, systemic administration of 7-nitro indazole (7-NI; 50 mg/kg given ip 30 min before tacrine), a selective inhibitor of nNOS, prevented the expression of motor and electrocortical (ECoG) seizures and abolished neuronal cell death in the hippocampus. A lower dose (5 mg/kg ip) of 7-NI was ineffective. In conclusion, the present data support a role for abnormal nNOS expression in the mechanism which triggers limbic seizures and delayed excitotoxic damage in the hippocampus of rat.",
keywords = "7-nitro indazole, ENOS, LiCl, Neuronal death, NNOS, NO, Seizures, Tacrine",
author = "Giacinto Bagetta and Paoletti, {Anna Maria} and Aida Leta and {Del Duca}, Claudio and Robert Nistic{\`o} and D. Rotiroti and Corasaniti, {M. Tiziana}",
year = "2002",
doi = "10.1006/bbrc.2002.6424",
language = "English",
volume = "291",
pages = "255--260",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Abnormal expression of neuronal nitric oxide synthase triggers limbic seizures and hippocampal damage in rat

AU - Bagetta, Giacinto

AU - Paoletti, Anna Maria

AU - Leta, Aida

AU - Del Duca, Claudio

AU - Nisticò, Robert

AU - Rotiroti, D.

AU - Corasaniti, M. Tiziana

PY - 2002

Y1 - 2002

N2 - Administration of tacrine (5 mg/kg ip), an anticho-linesterase agent, in rats pretreated (24 h beforehand) with lithium chloride (LiCl; 12 mEq/kg ip) provides a useful experimental model to study limbic seizures and delayed hippocampal damage. Here we report Western blotting evidence demonstrating that in rat LiCl and tacrine enhance the expression of neuronal nitric oxide synthase (nNOS), but not eNOS, enzyme protein in the hippocampus during the preconvulsive period and this triggers seizures and hippocampal damage. In fact, systemic administration of 7-nitro indazole (7-NI; 50 mg/kg given ip 30 min before tacrine), a selective inhibitor of nNOS, prevented the expression of motor and electrocortical (ECoG) seizures and abolished neuronal cell death in the hippocampus. A lower dose (5 mg/kg ip) of 7-NI was ineffective. In conclusion, the present data support a role for abnormal nNOS expression in the mechanism which triggers limbic seizures and delayed excitotoxic damage in the hippocampus of rat.

AB - Administration of tacrine (5 mg/kg ip), an anticho-linesterase agent, in rats pretreated (24 h beforehand) with lithium chloride (LiCl; 12 mEq/kg ip) provides a useful experimental model to study limbic seizures and delayed hippocampal damage. Here we report Western blotting evidence demonstrating that in rat LiCl and tacrine enhance the expression of neuronal nitric oxide synthase (nNOS), but not eNOS, enzyme protein in the hippocampus during the preconvulsive period and this triggers seizures and hippocampal damage. In fact, systemic administration of 7-nitro indazole (7-NI; 50 mg/kg given ip 30 min before tacrine), a selective inhibitor of nNOS, prevented the expression of motor and electrocortical (ECoG) seizures and abolished neuronal cell death in the hippocampus. A lower dose (5 mg/kg ip) of 7-NI was ineffective. In conclusion, the present data support a role for abnormal nNOS expression in the mechanism which triggers limbic seizures and delayed excitotoxic damage in the hippocampus of rat.

KW - 7-nitro indazole

KW - ENOS

KW - LiCl

KW - Neuronal death

KW - NNOS

KW - NO

KW - Seizures

KW - Tacrine

UR - http://www.scopus.com/inward/record.url?scp=0036293870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036293870&partnerID=8YFLogxK

U2 - 10.1006/bbrc.2002.6424

DO - 10.1006/bbrc.2002.6424

M3 - Article

C2 - 11846398

AN - SCOPUS:0036293870

VL - 291

SP - 255

EP - 260

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -