Abnormal pH-sensing of platelet NA+/H+ exchanger in patients with cardiac syndrome X

Erica De Candia, Gaetano Antonio Lanza, Enrico Romagnoli, Giovanni Ciabattoni, Alfonso Sestito, Patrizio Pasqualetti, Filippo Crea, Attilio Maseri, Raffaele Landolfi

Research output: Contribution to journalArticle

Abstract

An enhanced activity of Na+/Li+ countertransport, studied as a surrogate of Na+/H+ exchanger, has been described in red blood cells of patients with cardiac syndrome X. In this study we investigated whether abnormalities in the activity of platelet Na +/H+ exchanger (NHE) also existed in syndrome X patients and whether such abnormality was associated with platelet activation. Platelet NHE activity was evaluated in 21 syndrome X patients and 18 controls by measuring the pH recovery in platelets after acid loading and/or thrombin stimulation. The linear correlation existing between the initial intracytoplasmic pH (pHi) values and the maximal velocity of pH recovery allowed to calculate the values of slope and intercept at pH i=6.6 (IpH6.6) for each individual. Urinary excretion of the major TXB2 metabolite, 11-dehydro-TXB2 was measured in 15 syndrome X patients and 15 controls. The acidification-induced NHE activity resulted significantly higher in syndrome X patients compared to controls. Indeed, slope values were 0.75±0.29 and 0.5±0.23 min-1 in patients and controls, respectively (P=0.01), while IpH6.6 values were 0.24±0.1 and 0.17±0.1 ΔpH/min (P=0.04). The thrombin-stimulated NHE activity, however, was not different in the two groups and no significant difference in the urinary excretion of 11-dehydro-TXB 2 between patients and controls (median 920 vs. 765 pg/mg creatinine, respectively) (P=0.32) was also found. Thus our data demonstrate an alkaline shift in pH-dependence of platelet NHE of syndrome X patients. This abnormality does not seem to be associated with increased platelet activation.

Original languageEnglish
Pages (from-to)371-376
Number of pages6
JournalInternational Journal of Cardiology
Volume100
Issue number3
DOIs
Publication statusPublished - Apr 28 2005

Keywords

  • Na/Hexchanger
  • Platelets
  • Syndrome X

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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