Abnormal properties of skin fibroblasts from patients with breast cancer

B. Azzarone, M. Mareel, C. Billard, P. Scemama, C. Chaponnier, A. Macieira-Coelho

Research output: Contribution to journalArticle

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Abstract

The growth properties of fibroblasts from the thoracic skin of patients with mammary cancers were compared to those of fibroblastic cultures from patients with benign lesions or having undergone surgery for non-neoplastic diseases. As expected, an inverse correlation was found between the doubling potential of fibroblasts in vitro and the donor's age for cells from patients with benign lesions; however no correlation, was found with cultures from cancer patients. Moreover, the latter group responded in an abnormal way to three biological parameters: anchorage dependence, colony formation on monolayers of normal human epithelial cells and saturation densities in overcroweded culture conditions. Skin fibroblasts from one patient with a benign lesion, whose mother had developed a breast cancer, displayed all the abnormal growth properties. Periodic controls of this patient resulted in the early detection of a carcinoma 3 years after the first operation for a benign microcystic lesion. Finally, we found that multiple subcultivations in overcrowded culture conditions cause the selection of a fibroblastic cell subset with greater growth potential which, in the cell strain tested, could invade foreigh tissus in vitro.

Original languageEnglish
Pages (from-to)759-764
Number of pages6
JournalInternational Journal of Cancer
Volume33
Issue number6
Publication statusPublished - 1984

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Fibroblasts
Breast Neoplasms
Skin
Growth
Thorax
Cell Count
Epithelial Cells
Mothers
Tissue Donors
Carcinoma
Neoplasms
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Azzarone, B., Mareel, M., Billard, C., Scemama, P., Chaponnier, C., & Macieira-Coelho, A. (1984). Abnormal properties of skin fibroblasts from patients with breast cancer. International Journal of Cancer, 33(6), 759-764.

Abnormal properties of skin fibroblasts from patients with breast cancer. / Azzarone, B.; Mareel, M.; Billard, C.; Scemama, P.; Chaponnier, C.; Macieira-Coelho, A.

In: International Journal of Cancer, Vol. 33, No. 6, 1984, p. 759-764.

Research output: Contribution to journalArticle

Azzarone, B, Mareel, M, Billard, C, Scemama, P, Chaponnier, C & Macieira-Coelho, A 1984, 'Abnormal properties of skin fibroblasts from patients with breast cancer', International Journal of Cancer, vol. 33, no. 6, pp. 759-764.
Azzarone B, Mareel M, Billard C, Scemama P, Chaponnier C, Macieira-Coelho A. Abnormal properties of skin fibroblasts from patients with breast cancer. International Journal of Cancer. 1984;33(6):759-764.
Azzarone, B. ; Mareel, M. ; Billard, C. ; Scemama, P. ; Chaponnier, C. ; Macieira-Coelho, A. / Abnormal properties of skin fibroblasts from patients with breast cancer. In: International Journal of Cancer. 1984 ; Vol. 33, No. 6. pp. 759-764.
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