Abnormalities of membrane protein composition in patients with autoimmune haemolytic anaemia

V. De Angelis, M. C. De Matteis, M. R. Cozzi, F. Fiorin, P. Pradella, A. Steffan, L. Vettore

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Acquired abnormalities of red cell membrane protein composition in 37 patients with a positive direct antiglobulin test have been studied: 17 patients had true autoimmune haemolytic anaemia and 20 were HIV-infected subjects with a positive direct antiglobulin test but without signs of haemolysis. The study was carried out by performing sodium dodecyl sulphate polyacrylamide gel electrophoresis of ghost proteins followed by densitometric evaluation of the areas under the peaks, normalized by the total (α + β) spectrin content. Results show a significant decrease of bands 3, 4.1 and 4.2 over spectrin in patients with autoimmune haemolysis os compared to controls; at least in a small subset of patients, different specificities recognized by autoantibodies do not seem to account for these abnormalities which are reproducible independently from the molecular size of bands immunoprecipitated by autoantibodies. A similar decrease of protein 4.2 but not of band 3 staining intensity is also noticeable in HIV patients with a positive direct antiglobulin test. These results are consistent with the hypothesis that, following interactions between autoantibodies and autoantigens, modifications occur on membrane proteins resembling a variety of quantitative defects described in inherited haemolytic anaemias, and mainly the 'vertical interaction defects' of hereditary spherocytosis. Moreover, the decrease of band 3 staining intensity seems to represent a feature of patients with immune mediated haemolysis and not only with autoantibody binding.

Original languageEnglish
Pages (from-to)273-277
Number of pages5
JournalBritish Journal of Haematology
Volume95
Issue number2
Publication statusPublished - 1996

Fingerprint

Autoimmune Hemolytic Anemia
Membrane Proteins
Coombs Test
Autoantibodies
Hemolysis
Spectrin
HIV
Hereditary Spherocytosis
Staining and Labeling
Hemolytic Anemia
Autoantigens
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Proteins

Keywords

  • Autoimmune haemolytic anaemia
  • Band 3
  • HIV
  • Red cell membrane

ASJC Scopus subject areas

  • Hematology

Cite this

De Angelis, V., De Matteis, M. C., Cozzi, M. R., Fiorin, F., Pradella, P., Steffan, A., & Vettore, L. (1996). Abnormalities of membrane protein composition in patients with autoimmune haemolytic anaemia. British Journal of Haematology, 95(2), 273-277.

Abnormalities of membrane protein composition in patients with autoimmune haemolytic anaemia. / De Angelis, V.; De Matteis, M. C.; Cozzi, M. R.; Fiorin, F.; Pradella, P.; Steffan, A.; Vettore, L.

In: British Journal of Haematology, Vol. 95, No. 2, 1996, p. 273-277.

Research output: Contribution to journalArticle

De Angelis, V, De Matteis, MC, Cozzi, MR, Fiorin, F, Pradella, P, Steffan, A & Vettore, L 1996, 'Abnormalities of membrane protein composition in patients with autoimmune haemolytic anaemia', British Journal of Haematology, vol. 95, no. 2, pp. 273-277.
De Angelis, V. ; De Matteis, M. C. ; Cozzi, M. R. ; Fiorin, F. ; Pradella, P. ; Steffan, A. ; Vettore, L. / Abnormalities of membrane protein composition in patients with autoimmune haemolytic anaemia. In: British Journal of Haematology. 1996 ; Vol. 95, No. 2. pp. 273-277.
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