Abscisic acid activates the murine microglial cell line N9 through the second messenger cyclic ADP-ribose

Nicoletta Bodrato, Luisa Franco, Chiara Fresia, Lucrezia Guida, Cesare Usai, Annalisa Salis, Iliana Moreschi, Chiara Ferraris, Claudia Verderio, Giovanna Basile, Santina Bruzzone, Sonia Scarfi, Antonio De Flora, Elena Zocchi

Research output: Contribution to journalArticle

Abstract

Abscisic acid (ABA) is a phytohormone regulating important functions in higher plants, notably responses to abiotic stress. Recently, chemical or physical stimulation of human granulocytes was shown to induce production and release of endogenous ABA, which activates specific cell functions. Here we provide evidence that ABA stimulates several functional activities of the murine microglial cell line N9 (NO and tumor necrosis factor-a production, cell migration) through the second messenger cyclic ADP-ribose and an increase of intracellular calcium. ABA production and release occur in N9 cells stimulated with bacterial lipopolysaccharide, phorbol myristate acetate, the chemoattractant peptide f-MLP, or β-amyloid, the primary plaque component in Alzheimer disease. Finally, ABA priming stimulates N9 cell migration toward b-amyloid. These results indicate that ABA is a pro-inflammatory hormone inducing autocrine microglial activation, potentially representing a new target for anti-inflammatory therapies aimed at limiting microglia-induced tissue damage in the central nervous system.

Original languageEnglish
Pages (from-to)14777-14787
Number of pages11
JournalJournal of Biological Chemistry
Volume284
Issue number22
DOIs
Publication statusPublished - May 29 2009

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Bodrato, N., Franco, L., Fresia, C., Guida, L., Usai, C., Salis, A., Moreschi, I., Ferraris, C., Verderio, C., Basile, G., Bruzzone, S., Scarfi, S., De Flora, A., & Zocchi, E. (2009). Abscisic acid activates the murine microglial cell line N9 through the second messenger cyclic ADP-ribose. Journal of Biological Chemistry, 284(22), 14777-14787. https://doi.org/10.1074/jbc.M802604200