Absence of central nervous system pathology in severe combined immunodeficiency mice intraperitoneally injected with peripheral blood lymphocytes from multiple sclerosis patients

Gianvito Martino, Roberto Furlan, Elena Brambilla, Marina Castellano, Maria Rosa Terreni, Giancarlo Comi, Luigi M E Grimaldi

Research output: Contribution to journalArticlepeer-review

Abstract

In order to reproduce some of the pathological features of multiple sclerosis (MS) we transplanted peripheral blood lymphocytes (PBLs) from seven patients with MS into the peritoneal activity of 28 severe combined immunodeficiency (MS-SCID) mice. Seven SCID mice were also transplanted with PBLs from two healthy subjects (hu-SCID). Animals were sacrificed between 2 and 8 weeks after transplantation (a.t.). Polymerase chain reaction(PCR) using primers able to amplify the HLA-DQα region showed presence of human cells in neural tissues of MS-SCID mice. Immunocytochemical analysis revealed the scattered appearance of human lymphocytes (mostly CD45RO+ T cells) in the meningeal space and choroid plexuses of MS-SCID brains. However, human lymphocytes were similarly found in brains of hu-SCID mice. Both groups of mice never showed signs or symptoms of neurological impairment. Our results indicate that the simple transplantation of lymphocytes from MS patients into SCID mice is not likely to produce an MS-like pathology.

Original languageEnglish
Pages (from-to)213-217
Number of pages5
JournalJournal of Neuroimmunology
Volume55
Issue number2
DOIs
Publication statusPublished - 1994

Keywords

  • Animal models
  • Multiple sclerosis
  • Severe combined immunodeficiency mice

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

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