Absence of endogenous interleukin-10 enhances the evolution of acute lung injury

Salvatore Cuzzocrea, Emanuela Mazzon, Laura Dugo, Ivana Serraino, Rosanna Di Paola, Tiziana Genovese, Angela De Sarro, Achille P. Caputi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Interleukin-10 (IL-10) exerts a wide spectrum of regulatory activities in the immune and inflammatory response. The aim of this study was to investigate the role of endogenous IL-10 on the modulation of the inflammatory response in mice subjected to carrageenan-induced lung injury. When compared to carrageenan-treated IL-10 wild-type (WT) mice, carrageenan-treated IL-10 knock-out mice (IL-10KO) mice experienced a higher rate of pleural exudation, and polymorphonuclear cell migration. Exudate levels of the proinflammatory cytokines tumour necrosis factor, interleukin-1β and interleukin-6 were also greatly enhanced in IL-10KO mice in comparison to wild-type mice. Lung myeloperoxidase (MPO) activity was significantly reduced in IL-10WT mice when compared to IL-10KO mice-treated with carrageenan. The degree of oxidative and nitrosative damage was significantly higher in IL-10KO mice than in wild-type littermates, as indicated by elevated malondialdehyde levels and formation of nitrotyrosine and poly (ADP-ribose) synthetase (PARS). Staining of lung tissue sections obtained from carrageenan-treated IL-10WT with an anti-COX-2 antibody showed a positive staining of the inflamed tissue. Furthermore, expression of inducible nitric oxide synthase (iNOS) was found mainly in the macrophages of the inflamed lungs from carrageenan-treated IL-10WT mice. The intensity and degree of the staining for COX-2 and iNOS were markedly enhanced in tissue sections obtained from carrageenan-treated IL-10KO mice. Most notably, the degree of lung injury caused by carrageenan was also enhanced in IL-10KO mice. Taken together, our results clearly demonstrate that endogenous IL-10 exerts an anti-inflammatory role during acute inflammation and tissue damage associated with carrageenan-induced pleurisy, possibly by regulating neutrophil recruitment, and the subsequent cytokine and oxidant generation.

Original languageEnglish
Pages (from-to)285-297
Number of pages13
JournalEuropean Cytokine Network
Volume13
Issue number3
Publication statusPublished - Jul 2002

Fingerprint

Acute Lung Injury
Carrageenan
Interleukin-10
Knockout Mice
Tissue
Nitric Oxide Synthase Type II
Lung Injury
Staining and Labeling
Lung
Cytokines
Poly Adenosine Diphosphate Ribose
Macrophages
Pleurisy
Neutrophil Infiltration
Ligases
Malondialdehyde
Interleukin-1
Oxidants
Exudates and Transudates
Peroxidase

Keywords

  • Carrageenan
  • IL-10
  • Inflammation
  • Nitric oxide
  • Peroxynitrite
  • Superoxide dismutase

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Cuzzocrea, S., Mazzon, E., Dugo, L., Serraino, I., Di Paola, R., Genovese, T., ... Caputi, A. P. (2002). Absence of endogenous interleukin-10 enhances the evolution of acute lung injury. European Cytokine Network, 13(3), 285-297.

Absence of endogenous interleukin-10 enhances the evolution of acute lung injury. / Cuzzocrea, Salvatore; Mazzon, Emanuela; Dugo, Laura; Serraino, Ivana; Di Paola, Rosanna; Genovese, Tiziana; De Sarro, Angela; Caputi, Achille P.

In: European Cytokine Network, Vol. 13, No. 3, 07.2002, p. 285-297.

Research output: Contribution to journalArticle

Cuzzocrea, S, Mazzon, E, Dugo, L, Serraino, I, Di Paola, R, Genovese, T, De Sarro, A & Caputi, AP 2002, 'Absence of endogenous interleukin-10 enhances the evolution of acute lung injury', European Cytokine Network, vol. 13, no. 3, pp. 285-297.
Cuzzocrea S, Mazzon E, Dugo L, Serraino I, Di Paola R, Genovese T et al. Absence of endogenous interleukin-10 enhances the evolution of acute lung injury. European Cytokine Network. 2002 Jul;13(3):285-297.
Cuzzocrea, Salvatore ; Mazzon, Emanuela ; Dugo, Laura ; Serraino, Ivana ; Di Paola, Rosanna ; Genovese, Tiziana ; De Sarro, Angela ; Caputi, Achille P. / Absence of endogenous interleukin-10 enhances the evolution of acute lung injury. In: European Cytokine Network. 2002 ; Vol. 13, No. 3. pp. 285-297.
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