Absence of Fhit protein in primary lung tumors and cell lines with FHIT gene abnormalities

Gabriella Sozzi, Silvana Tornielli, Elda Tagliabue, Laura Sard, Francesco Pezzella, Ugo Pastorino, Fabiola Minoletti, Silvana Pilotti, Cathy Ratcliffe, Maria Luisa Veronese, Peter Goldstraw, Kay Huebner, Carlo M. Croce, Marco A. Pierotti

Research output: Contribution to journalArticlepeer-review

Abstract

Genomic alterations and abnormal expression of the FHIT gene at 3p14.2 have been observed in cell lines and primary tumors of the lung. To correlate FHIT locus DNA and RNA lesions with effects on Fhit protein expression, we have analyzed 11 lung cancer lines, 15 small cell lung carcinomas, and 38 pairs of non-small cell primary tumors and bronchial mucosa specimens by molecular genetic immunocytochemical methods. Using specific antibodies against the Fhit protein, we observed concordance between RNA abnormalities and lack of Fhit protein expression in lung tumors and cell lines. In addition, absence of Fhit protein in some precancerous dysplastic lesions suggested that FHIT inactivation may occur at an early phase of lung carcinogenesis.

Original languageEnglish
Pages (from-to)5207-5212
Number of pages6
JournalCancer Research
Volume57
Issue number23
Publication statusPublished - Dec 1 1997

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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