Absence of HTLV-I/II infection in blood donors with positive and inconclusive HTLV-I/II serology.

M. C. Re, G. Furlini, E. Ramazzotti, M. Vignoli, G. Zauli, S. Lolli, P. Monari, D. Belletti, A. Nanetti, M. La Placa

Research output: Contribution to journalArticle

Abstract

The pathogenetic potential and the true extent of human T leukemia/lymphotropic virus type I (HTLV-I) and type II (HTLV-II) infection are unknown. To find out more about HTLV-I/II seroepidemiology and the risks of iatrogenic transmission, we performed a serological study, screening 4086 healthy blood donors. A surprisingly high percentage of serum reactivity to HTLV-I/II antigens was observed by commercial ELISA (2.08%) and immunoblotting (IB) (0.85%) analysis, although none of the samples satisfied the (IB) criteria for positivity based on detection of gag protein p24 and at least one env gene product, either gp46 or gp61/68. To clarify these inconclusive results, we performed polymerase chain reaction (PCR) analysis for HTLV-I and HTLV-II provirus detection in peripheral blood lymphocytes, obtained from individuals with an apparent pattern of seropositivity. The data obtained by PCR failed to reveal evidence of HTLV-I/II provirus integration in peripheral blood cells, ruling out the possibility of a viral infection in these cases, and pinpointing the limitations of both serological methods used. Our observations suggest that serological assays alone are not a reliable tool for blood donor screening of HTLV-I/II infection and raise the important question of interpreting inconclusive results.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalMicrobiologica
Volume15
Issue number4
Publication statusPublished - Oct 1992

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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    Re, M. C., Furlini, G., Ramazzotti, E., Vignoli, M., Zauli, G., Lolli, S., Monari, P., Belletti, D., Nanetti, A., & La Placa, M. (1992). Absence of HTLV-I/II infection in blood donors with positive and inconclusive HTLV-I/II serology. Microbiologica, 15(4), 329-336.