Absence of microsatellite instability in breast carcinomas with both p53 and c-erbB-2 alterations

Cristina Formantici, Rosaria Orlandi, Chiara Ronchini, Silvana Pilotti, Guglielmina N. Ranzani, Maria I. Colnaghi, Sylvie Ménard

Research output: Contribution to journalArticlepeer-review

Abstract

Based on a previous finding that amplification of the c-erbB-2 oncogene and alteration of p53 are strongly associated in most aggressive breast tumours, the present study investigated whether microsatellite instability (MI) might also be associated with this tumour phenotype. Nine polymorphic microsatellite markers, including six dinucleotide, one trinucleotide, and two tetranucleotide repeats, were amplified from paired normal and tumour DNA samples of 15 breast tumours that overexpressed both c-erbB-2 and p53 and of 15 control breast tumours that overexpressed neither protein. All 30 breast tumours analysed exhibited a replication error-negative phenotype, with only one sample showing MI in one of the nine loci. This suggests that the genetic events underlying MI, which are critical in colorectal and gastric tumours, are not involved in the pathogenesis of c-erbB-2/p53 double-altered breast tumours and do not play a central role in breast tumour formation.

Original languageEnglish
Pages (from-to)424-427
Number of pages4
JournalJournal of Pathology
Volume187
Issue number4
DOIs
Publication statusPublished - 1999

Keywords

  • Breast carcinoma
  • c-erbB-2
  • Microsatellite instability
  • p53
  • PCR

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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