Abstract
Pancreatic cancers frequently have defects in components of the transforming growth factor-β (TGF-β) signaling pathway. TIEG1 (TGF-β inducible early gene) is a recently characterized transcription factor regulated by TGF-β that induces apoptosis when overexpressed in pancreatic adenocarcinoma cell lines. Alterations on chromosome 8q, where TIEG1 is located, are also relatively frequent in pancreatic cancers. To determine if TIEG1 may be involved in the tumorigenesis of pancreatic cancer, we performed mutational screening of this gene in 22 pancreatic cancer cell lines. No sequence alterations were observed. Reverse transcription-polymerase chain reaction analysis was also performed to rule out the possibility that the expression of the gene is altered by genetic events other than mutation. Likewise, no alterations in expression were found. Thus, an essential role of TIEG1 in pancreatic cancer can be excluded.
| Original language | English |
|---|---|
| Pages (from-to) | 179-183 |
| Number of pages | 5 |
| Journal | Cancer Letters |
| Volume | 183 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Sep 26 2002 |
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Keywords
- Apoptosis
- Cancer
- Pancreas
- TIEG1
- Transforming growth factor β
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology
Cite this
Absence of mutations in the transforming growth factor-β inducible early gene 1, TIEG1, in pancreatic cancer. / Antonello, Davide; Moore, Patrick S.; Zamboni, Giuseppe; Falconi, Massimo; Scarpa, Aldo.
In: Cancer Letters, Vol. 183, No. 2, 26.09.2002, p. 179-183.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Absence of mutations in the transforming growth factor-β inducible early gene 1, TIEG1, in pancreatic cancer
AU - Antonello, Davide
AU - Moore, Patrick S.
AU - Zamboni, Giuseppe
AU - Falconi, Massimo
AU - Scarpa, Aldo
PY - 2002/9/26
Y1 - 2002/9/26
N2 - Pancreatic cancers frequently have defects in components of the transforming growth factor-β (TGF-β) signaling pathway. TIEG1 (TGF-β inducible early gene) is a recently characterized transcription factor regulated by TGF-β that induces apoptosis when overexpressed in pancreatic adenocarcinoma cell lines. Alterations on chromosome 8q, where TIEG1 is located, are also relatively frequent in pancreatic cancers. To determine if TIEG1 may be involved in the tumorigenesis of pancreatic cancer, we performed mutational screening of this gene in 22 pancreatic cancer cell lines. No sequence alterations were observed. Reverse transcription-polymerase chain reaction analysis was also performed to rule out the possibility that the expression of the gene is altered by genetic events other than mutation. Likewise, no alterations in expression were found. Thus, an essential role of TIEG1 in pancreatic cancer can be excluded.
AB - Pancreatic cancers frequently have defects in components of the transforming growth factor-β (TGF-β) signaling pathway. TIEG1 (TGF-β inducible early gene) is a recently characterized transcription factor regulated by TGF-β that induces apoptosis when overexpressed in pancreatic adenocarcinoma cell lines. Alterations on chromosome 8q, where TIEG1 is located, are also relatively frequent in pancreatic cancers. To determine if TIEG1 may be involved in the tumorigenesis of pancreatic cancer, we performed mutational screening of this gene in 22 pancreatic cancer cell lines. No sequence alterations were observed. Reverse transcription-polymerase chain reaction analysis was also performed to rule out the possibility that the expression of the gene is altered by genetic events other than mutation. Likewise, no alterations in expression were found. Thus, an essential role of TIEG1 in pancreatic cancer can be excluded.
KW - Apoptosis
KW - Cancer
KW - Pancreas
KW - TIEG1
KW - Transforming growth factor β
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UR - http://www.scopus.com/inward/citedby.url?scp=0037179546&partnerID=8YFLogxK
U2 - 10.1016/S0304-3835(01)00802-3
DO - 10.1016/S0304-3835(01)00802-3
M3 - Article
C2 - 12065093
AN - SCOPUS:0037179546
VL - 183
SP - 179
EP - 183
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 2
ER -