Absence of nucleolar disruption after impairment of 40S ribosome biogenesis reveals an rpL11-translationdependent mechanism of p53 induction

Stefano Fumagalli, Alessandro Di Cara, Arti Neb-Gulati, Francois Natt, Sandy Schwemberger, Jonathan Hall, George F. Babcock, Rosa Bernardi, Pier Paolo Pandolfi, George Thomas

Research output: Contribution to journalArticle

Abstract

Impaired ribosome biogenesis is attributed to nucleolar disruption and diffusion of a subset of 60S ribosomal proteins, particularly ribosomal protein (rp)L11, into the nucleoplasm, where they inhibit MDM2, leading to p53 induction and cellcycle arrest1-4. Previously, we demonstrated that deletion of the 40S rpS6 gene in mouse liver prevents hepatocytes from re-entering the cell cycle after partial hepatectomy5. Here, we show that this response leads to an increase in p53, which is recapitulated in culture by rpS6-siRNA treatment and rescued by the simultaneous depletion of p53. However, disruption of biogenesis of 40S ribosomes had no effect on nucleolar integrity, although p53 induction was mediated by rpL11, leading to the finding that the cell selectively upregulates the translation of mRNAs with a polypyrimidine tract at their 5′-transcriptional start site (5′-TOP mRNAs), including that encoding rpL11, on impairment of 40S ribosome biogenesis. Increased 5′-TOP mRNA translation takes place despite continued 60S ribosome biogenesis and a decrease in global translation. Thus, in proliferative human disorders involving hypomorphic mutations in 40S ribosomal proteins6,7, specific targeting of rpL11 upregulation would spare other stress pathways that mediate the potential benefits of p53 induction8.

Original languageEnglish
Pages (from-to)501-508
Number of pages8
JournalNature Cell Biology
Volume11
Issue number4
DOIs
Publication statusPublished - 2009

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ASJC Scopus subject areas

  • Cell Biology

Cite this

Fumagalli, S., Di Cara, A., Neb-Gulati, A., Natt, F., Schwemberger, S., Hall, J., Babcock, G. F., Bernardi, R., Pandolfi, P. P., & Thomas, G. (2009). Absence of nucleolar disruption after impairment of 40S ribosome biogenesis reveals an rpL11-translationdependent mechanism of p53 induction. Nature Cell Biology, 11(4), 501-508. https://doi.org/10.1038/ncb1858