Absence of the cell cycle inhibitor p27(Kip1) protein predicts poor outcome in patients with stage I-III colorectal cancer

Claudio Belluco, Giovanni Esposito, Roberta Bertorelle, Annarosa Del Mistro, Ambrogio Fassina, Giulia Vieceli, Luigi Chieco-Bianchi, Donato Nitti, Mario Lise

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: The p27(Kip1) protein regulates the G1 to S phase transition of cell cycle by binding to and inhibiting the cyclin E/Cdk2 complex. This study explores the prognostic significance of the absence of the p27(Kip1) protein in patients with colorectal cancer (CRC). Methods: Formalin-fixed tumor sections from 124 patients who underwent curative resection for stage I-III CRC were analyzed by immunohistochemistry using MoAb anti-p27(Kip1). Results: Detectable levels of p27(Kip1) protein were found in 86% of tumors. Median follow-up was 55 months. Actuarial 5-year disease-free survival (DFS) and overall survival (OS) were 76% and 85%, respectively, in patients with tumors with p27(Kip1) protein expression and 34% and 40%, respectively, in those whose tumors lacked p27(Kip1) protein expression (P <.001). At multivariate analysis, tumor stage (III vs. I-II) and p27(Kip1) protein status (absence vs. presence) were found to be independent prognostic factors for DFS and OS. Conclusions: Lack of p27(Kip1) protein expression in CRC is a negative prognostic marker and may therefore be useful in selecting early- stage patients more likely to benefit from adjuvant treatment.

Original languageEnglish
Pages (from-to)19-25
Number of pages7
JournalAnnals of Surgical Oncology
Volume6
Issue number1
DOIs
Publication statusPublished - Jan 1999

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Cyclin-Dependent Kinase Inhibitor p27
Colorectal Neoplasms
Cell Cycle
Neoplasms
Disease-Free Survival
Cyclin E
Survival
Status Epilepticus
Phase Transition
S Phase
Formaldehyde
Multivariate Analysis
Immunohistochemistry

Keywords

  • Cell cycle inhibitors.
  • Colorectal carcinoma
  • p27(Kip1) protein
  • Prognostic markers

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

Absence of the cell cycle inhibitor p27(Kip1) protein predicts poor outcome in patients with stage I-III colorectal cancer. / Belluco, Claudio; Esposito, Giovanni; Bertorelle, Roberta; Del Mistro, Annarosa; Fassina, Ambrogio; Vieceli, Giulia; Chieco-Bianchi, Luigi; Nitti, Donato; Lise, Mario.

In: Annals of Surgical Oncology, Vol. 6, No. 1, 01.1999, p. 19-25.

Research output: Contribution to journalArticle

Belluco, Claudio ; Esposito, Giovanni ; Bertorelle, Roberta ; Del Mistro, Annarosa ; Fassina, Ambrogio ; Vieceli, Giulia ; Chieco-Bianchi, Luigi ; Nitti, Donato ; Lise, Mario. / Absence of the cell cycle inhibitor p27(Kip1) protein predicts poor outcome in patients with stage I-III colorectal cancer. In: Annals of Surgical Oncology. 1999 ; Vol. 6, No. 1. pp. 19-25.
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AU - Esposito, Giovanni

AU - Bertorelle, Roberta

AU - Del Mistro, Annarosa

AU - Fassina, Ambrogio

AU - Vieceli, Giulia

AU - Chieco-Bianchi, Luigi

AU - Nitti, Donato

AU - Lise, Mario

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