Absence of TI-VAMP/Vamp7 leads to increased anxiety in Mice

Lydia Danglot, Kathleen Zylbersztejn, Maja Petkovic, Maxime Gauberti, Hamid Meziane, Roy Combe, Marie France Champy, Marie Christine Birling, Guillaume Pavlovic, Jean Charles Bizot, Fabrice Trovero, Floriana Della Ragione, Véronique Proux-Gillardeaux, Tania Sorg, Denis Vivien, Maurizio D'Esposito, Thierry Galli

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Vesicular (v)- and target (t)-SNARE proteins assemble in SNARE complex to mediate membrane fusion. Tetanus neurotoxin-insensitive vesicular-associated membrane protein (TI-VAMP/VAMP7), a vesicular SNARE expressed in several cell types including neurons, was previously shown to play a major role in exocytosis involved in neurite growth in cultured neurons. Here we generated a complete constitutive knock-out by deleting the exon 3 of Vamp7. Loss of TI-VAMP expression did not lead to any striking developmental or neurological defect. Knock-out mice displayed decreased brain weight and increased third ventricle volume. Axon growth appeared normal in cultured knock-out neurons. Behavioral characterization unraveled that TI-VAMP knock-out was associated with increased anxiety. Our results thus suggest compensatory mechanisms allowing the TI-VAMP knock-out mice to fulfill major developmental processes. The phenotypic traits unraveled here further indicate an unexpected role of TI-VAMP-mediated vesicular traffic in anxiety and suggest a role for TI-VAMP in higher brain functions.

Original languageEnglish
Pages (from-to)1962-1968
Number of pages7
JournalJournal of Neuroscience
Volume32
Issue number6
DOIs
Publication statusPublished - Feb 8 2012

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SNARE Proteins
Anxiety
Neurons
Knockout Mice
Membrane Fusion
Third Ventricle
Exocytosis
Brain
Neurites
Growth
Axons
Exons
Membrane Proteins
Weights and Measures

ASJC Scopus subject areas

  • Neuroscience(all)

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Danglot, L., Zylbersztejn, K., Petkovic, M., Gauberti, M., Meziane, H., Combe, R., ... Galli, T. (2012). Absence of TI-VAMP/Vamp7 leads to increased anxiety in Mice. Journal of Neuroscience, 32(6), 1962-1968. https://doi.org/10.1523/JNEUROSCI.4436-11.2012

Absence of TI-VAMP/Vamp7 leads to increased anxiety in Mice. / Danglot, Lydia; Zylbersztejn, Kathleen; Petkovic, Maja; Gauberti, Maxime; Meziane, Hamid; Combe, Roy; Champy, Marie France; Birling, Marie Christine; Pavlovic, Guillaume; Bizot, Jean Charles; Trovero, Fabrice; Ragione, Floriana Della; Proux-Gillardeaux, Véronique; Sorg, Tania; Vivien, Denis; D'Esposito, Maurizio; Galli, Thierry.

In: Journal of Neuroscience, Vol. 32, No. 6, 08.02.2012, p. 1962-1968.

Research output: Contribution to journalArticle

Danglot, L, Zylbersztejn, K, Petkovic, M, Gauberti, M, Meziane, H, Combe, R, Champy, MF, Birling, MC, Pavlovic, G, Bizot, JC, Trovero, F, Ragione, FD, Proux-Gillardeaux, V, Sorg, T, Vivien, D, D'Esposito, M & Galli, T 2012, 'Absence of TI-VAMP/Vamp7 leads to increased anxiety in Mice', Journal of Neuroscience, vol. 32, no. 6, pp. 1962-1968. https://doi.org/10.1523/JNEUROSCI.4436-11.2012
Danglot L, Zylbersztejn K, Petkovic M, Gauberti M, Meziane H, Combe R et al. Absence of TI-VAMP/Vamp7 leads to increased anxiety in Mice. Journal of Neuroscience. 2012 Feb 8;32(6):1962-1968. https://doi.org/10.1523/JNEUROSCI.4436-11.2012
Danglot, Lydia ; Zylbersztejn, Kathleen ; Petkovic, Maja ; Gauberti, Maxime ; Meziane, Hamid ; Combe, Roy ; Champy, Marie France ; Birling, Marie Christine ; Pavlovic, Guillaume ; Bizot, Jean Charles ; Trovero, Fabrice ; Ragione, Floriana Della ; Proux-Gillardeaux, Véronique ; Sorg, Tania ; Vivien, Denis ; D'Esposito, Maurizio ; Galli, Thierry. / Absence of TI-VAMP/Vamp7 leads to increased anxiety in Mice. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 6. pp. 1962-1968.
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