Absolute quantification of cell-free microRNAs in cancer patients

Manuela Ferracin, Laura Lupini, Irene Salamon, Elena Saccenti, Maria Vittoria Zanzi, Andrea Rocchi, Lucia Da Ros, Barbara Zagatti, Gentian Musa, Cristian Bassi, Alessandra Mangolini, Giorgio Cavallesco, Antonio Frassoldati, Stefano Volpato, Paolo Carcoforo, Alan B. Hollingsworth, Massimo Negrini

Research output: Contribution to journalArticle

Abstract

The hypothesis to use microRNAs (miRNAs) circulating in the blood as cancer biomarkers was formulated some years ago based on promising initial results. After some exciting discoveries, however, it became evident that the accurate quantification of cell-free miRNAs was more challenging than expected. Difficulties were linked to the strong impact that many, if not all, pre- and post- analytical variables have on the final results. In this study, we used currently available high-throughput technologies to identify miRNAs present in plasma and serum of patients with breast, colorectal, lung, thyroid and melanoma tumors, and healthy controls. Then, we assessed the absolute level of nine different miRNAs (miR-320a, miR-21-5p, miR-378a-3p, miR-181a-5p, miR-3156-5p, miR-2110, miR-125a-5p, miR-425-5p, miR-766-3p) in 207 samples from healthy controls and cancer patients using droplet digital PCR (ddPCR) technology. We identified miRNAs specifically modulated in one or more cancer types, according to tissue source. The significant reduction of miR-181a-5p levels in breast cancer patients serum was further validated using two independent cohorts, one from Italy (n = 70) and one from US (n = 90), with AUC 0.66 and 0.73 respectively. This study finally powers the use of cell-free miRNAs as cancer biomarkers and propose miR-181a-5p as a diagnostic breast cancer biomarker.

Original languageEnglish
Pages (from-to)14545-14555
Number of pages11
JournalOncotarget
Volume6
Issue number16
Publication statusPublished - 2015

Keywords

  • Breast cancer
  • Cancer biomarkers
  • Cell-free microRNA
  • Droplet digital PCR

ASJC Scopus subject areas

  • Oncology

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  • Cite this

    Ferracin, M., Lupini, L., Salamon, I., Saccenti, E., Zanzi, M. V., Rocchi, A., Da Ros, L., Zagatti, B., Musa, G., Bassi, C., Mangolini, A., Cavallesco, G., Frassoldati, A., Volpato, S., Carcoforo, P., Hollingsworth, A. B., & Negrini, M. (2015). Absolute quantification of cell-free microRNAs in cancer patients. Oncotarget, 6(16), 14545-14555.