Absolute quantitation of viremia in human immunodeficiency virus infection by competitive reverse transcription and polymerase chain reaction

S. Menzo, P. Bagnarelli, M. Giacca, A. Manzin, P. E. Varaldo, M. Clementi

Research output: Contribution to journalArticle

Abstract

A competitive polymerase chain reaction (PCR)-based assay for the quantitative detection of human immunodeficiency virus type 1 (HIV-1) viremia was developed and optimized. This method consists of the reverse transcription and subsequent amplification in the same tube of two similar RNA templates, the wild-type template to be quantified and a known amount of the internally deleted synthetic template, both with identical primer recognition sites. The same strategy also proved to be useful in the quantitative assay of HIV-1-specific cellular transcripts and proviral DNA sequences from peripheral blood mononuclear cells by using competitor DNA. The method might be of interest in the study of the precise level of HIV-1 activity during the different clinical phases of the infection and in the simple, fast, and methodologically correct molecular investigation of patients treated with specific antiviral compounds.

Original languageEnglish
Pages (from-to)1752-1757
Number of pages6
JournalJournal of Clinical Microbiology
Volume30
Issue number7
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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