Absorption of bioactive compounds from steamed broccoli and their effect on plasma glutathione S-transferase activity

Patrizia Riso, Antonella Brusamolino, Massimo Moro, Marisa Porrini

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Cruciferous vegetables are characterized by high amounts of glucosinolates (GLSs) that are hydrolysed to isothiocyanates (ITCs) and other phytochemicals. The aim of the study was to verify the effect of broccoli intake on plasma levels of carotenoids, vitamins and ITCs and on glutathione S-transferase (GST) activity. Twenty healthy subjects, characterized for GSTM1 and GSTT1 genotype, participated in a cross-over intervention study (broccoli diet versus cruciferous-free diet). Subjects consumed a daily portion of broccoli (10 days, 200 g) providing glucosinolates (200 μmol non-indolyl ITCs evaluated by cyclocondensation reaction after myrosinase treatment), vitamin C (about 100 mg) and carotenoids (about 5 mg lutein and β-carotene). An increase of folate, carotenoids and ITC plasma concentrations was found. The increase of plasma ITC concentration was independent of the GST genotype. Broccoli intervention did not affect plasma GST activity. Broccoli is a bioavailable source of diverse compounds whose effects on endogenous defence systems deserve further investigation.

Original languageEnglish
Pages (from-to)56-71
Number of pages16
JournalInternational Journal of Food Sciences and Nutrition
Volume60
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2009

Fingerprint

Isothiocyanates
Brassica
isothiocyanates
broccoli
Glutathione Transferase
glutathione transferase
Carotenoids
Glucosinolates
carotenoids
glucosinolates
Genotype
Diet
thioglucosidase
Lutein
genotype
Phytochemicals
carotenes
lutein
Folic Acid
Vitamins

Keywords

  • Bioactive compounds
  • Biomarker
  • Humans
  • Nutritional intervention
  • Vegetables

ASJC Scopus subject areas

  • Food Science

Cite this

Absorption of bioactive compounds from steamed broccoli and their effect on plasma glutathione S-transferase activity. / Riso, Patrizia; Brusamolino, Antonella; Moro, Massimo; Porrini, Marisa.

In: International Journal of Food Sciences and Nutrition, Vol. 60, No. SUPPL. 1, 2009, p. 56-71.

Research output: Contribution to journalArticle

Riso, Patrizia ; Brusamolino, Antonella ; Moro, Massimo ; Porrini, Marisa. / Absorption of bioactive compounds from steamed broccoli and their effect on plasma glutathione S-transferase activity. In: International Journal of Food Sciences and Nutrition. 2009 ; Vol. 60, No. SUPPL. 1. pp. 56-71.
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