TY - JOUR
T1 - Absorption of bioactive compounds from steamed broccoli and their effect on plasma glutathione S-transferase activity
AU - Riso, Patrizia
AU - Brusamolino, Antonella
AU - Moro, Massimo
AU - Porrini, Marisa
PY - 2009
Y1 - 2009
N2 - Cruciferous vegetables are characterized by high amounts of glucosinolates (GLSs) that are hydrolysed to isothiocyanates (ITCs) and other phytochemicals. The aim of the study was to verify the effect of broccoli intake on plasma levels of carotenoids, vitamins and ITCs and on glutathione S-transferase (GST) activity. Twenty healthy subjects, characterized for GSTM1 and GSTT1 genotype, participated in a cross-over intervention study (broccoli diet versus cruciferous-free diet). Subjects consumed a daily portion of broccoli (10 days, 200 g) providing glucosinolates (200 μmol non-indolyl ITCs evaluated by cyclocondensation reaction after myrosinase treatment), vitamin C (about 100 mg) and carotenoids (about 5 mg lutein and β-carotene). An increase of folate, carotenoids and ITC plasma concentrations was found. The increase of plasma ITC concentration was independent of the GST genotype. Broccoli intervention did not affect plasma GST activity. Broccoli is a bioavailable source of diverse compounds whose effects on endogenous defence systems deserve further investigation.
AB - Cruciferous vegetables are characterized by high amounts of glucosinolates (GLSs) that are hydrolysed to isothiocyanates (ITCs) and other phytochemicals. The aim of the study was to verify the effect of broccoli intake on plasma levels of carotenoids, vitamins and ITCs and on glutathione S-transferase (GST) activity. Twenty healthy subjects, characterized for GSTM1 and GSTT1 genotype, participated in a cross-over intervention study (broccoli diet versus cruciferous-free diet). Subjects consumed a daily portion of broccoli (10 days, 200 g) providing glucosinolates (200 μmol non-indolyl ITCs evaluated by cyclocondensation reaction after myrosinase treatment), vitamin C (about 100 mg) and carotenoids (about 5 mg lutein and β-carotene). An increase of folate, carotenoids and ITC plasma concentrations was found. The increase of plasma ITC concentration was independent of the GST genotype. Broccoli intervention did not affect plasma GST activity. Broccoli is a bioavailable source of diverse compounds whose effects on endogenous defence systems deserve further investigation.
KW - Bioactive compounds
KW - Biomarker
KW - Humans
KW - Nutritional intervention
KW - Vegetables
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U2 - 10.1080/09637480802089751
DO - 10.1080/09637480802089751
M3 - Article
C2 - 19424928
AN - SCOPUS:68949191152
VL - 60
SP - 56
EP - 71
JO - International Journal of Food Sciences and Nutrition
JF - International Journal of Food Sciences and Nutrition
SN - 0963-7486
IS - SUPPL. 1
ER -