Accelerated dendritic-cell migration and T-cell priming in SPARC-deficient mice

Sabina Sangaletti, Lucia Gioiosa, Cristiana Guiducci, Gianlucca Rotta, Maria Rescigno, Antonella Stoppacciaro, Claudia Chiodoni, Mario P. Colombo

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

On their path to draining lymph nodes, epidermal Langerhans cells traverse collagen-dense connective tissue before reaching lymphatic vessels. The matricellular protein SPARC (secreted protein, acidic and rich in cysteine), which is induced during inflammation and tissue repair, organizes collagen deposition in tissue stroma. We analyzed Langerhans cell and dendritic-cell migration and its impact on T-cell priming in SPARC-null (SPARC-/-) and SPARC-sufficient (SPARC+/+) mice. Although the same number of Langerhans cells populate the ear skin of SPARC-/- and SPARC+/+ mice, more Langerhans cells were found in the lymph nodes draining antigen-sensitized ears of SPARC-/- mice and significantly more Langerhans cells migrated from null-mice-derived ear skin explants. Such favored Langerhans cell migration is due to the host environment, as demonstrated by SPARC+/+> SPARC-/- and reciprocal chimeras, and have a profound influence on T-cell priming. Contact-, delayed type-hypersensitivity and naive T-cell receptor-transgenic T-cell priming, together indicate that the lack of SPARC in the environment accelerates the onset of T-cell priming by hastening Langerhans cell/dendritic-cell migration.

Original languageEnglish
Pages (from-to)3685-3694
Number of pages10
JournalJournal of Cell Science
Volume118
Issue number16
DOIs
Publication statusPublished - Aug 15 2005

Fingerprint

Langerhans Cells
Dendritic Cells
Cell Movement
Cysteine
T-Lymphocytes
Proteins
Ear
Collagen
Lymph Nodes
Skin
Lymphatic Vessels
mouse SPARC protein
Contact Dermatitis
Delayed Hypersensitivity
T-Cell Antigen Receptor
Connective Tissue
Inflammation
Antigens

Keywords

  • DC migration
  • SPARC
  • T-cell priming

ASJC Scopus subject areas

  • Cell Biology

Cite this

Accelerated dendritic-cell migration and T-cell priming in SPARC-deficient mice. / Sangaletti, Sabina; Gioiosa, Lucia; Guiducci, Cristiana; Rotta, Gianlucca; Rescigno, Maria; Stoppacciaro, Antonella; Chiodoni, Claudia; Colombo, Mario P.

In: Journal of Cell Science, Vol. 118, No. 16, 15.08.2005, p. 3685-3694.

Research output: Contribution to journalArticle

Sangaletti S, Gioiosa L, Guiducci C, Rotta G, Rescigno M, Stoppacciaro A et al. Accelerated dendritic-cell migration and T-cell priming in SPARC-deficient mice. Journal of Cell Science. 2005 Aug 15;118(16):3685-3694. https://doi.org/10.1242/jcs.02474
Sangaletti, Sabina ; Gioiosa, Lucia ; Guiducci, Cristiana ; Rotta, Gianlucca ; Rescigno, Maria ; Stoppacciaro, Antonella ; Chiodoni, Claudia ; Colombo, Mario P. / Accelerated dendritic-cell migration and T-cell priming in SPARC-deficient mice. In: Journal of Cell Science. 2005 ; Vol. 118, No. 16. pp. 3685-3694.
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