Accelerated solvent extraction then liquid chromatography coupled with mass spectrometry for determination of 4-t-octylphenol, 4-nonylphenols, and bisphenol A in fish liver

S. Tavazzi, E. Benfenati, D. Barceló

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23 Citations (Scopus)

Abstract

Analysis of extracts from fish liver containing alkylphenol contaminants can be hindered by the presence of co-extracted fats ond proteins that interfere with chromatographic onalysis. In this study accelerated solvent extroction (ASE), Florisil clean-up, then combined liquid chromatography - mass spectrometry (LC-MS) with an electrospray (ESI) interface have been used to optimize an analytical procedure for the analysis of octylphenol, nonylphenol, and bisphenol A in fish liver. After comparison of the efficiency of ASE with conventionol Soxhlet extraction the developed procedure was applied to the analysis of liver samples. Calibration plots of the relationship between concentration and the ratio of the responses to the analyte and to the internol standard, 4-n-nonylphenol, were determined by linear regression analysis over the concentration range 0.05 to 10 ppm and resulted in good fits (r2 > 0.994). Recoveries (evaluated for each liver somple as the ratio between response to the surrogote compound, 4-n-nonylphenol, and that to the internal standard, 4-n-heptylphenol, relative to the some ratio for o reference standard solution) were 53 ± 20%. Under the experimentol conditions used in this work the limits of detection (LOD), calculated by use of a signal-to-noise ratio of 3: 1, were 5 ng g-1 for 4-t-octylphenol, 15 ng g-1 for bisphenol A, and 20 ng g-1 for nonylphenol. The method can be sotisfactorily applied to screening analysis of octyl- and nonylphenols and bisphenol A in biological samples such as fish liver.

Original languageEnglish
Pages (from-to)463-467
Number of pages5
JournalChromatographia
Volume56
Issue number7-8
Publication statusPublished - Oct 2002

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Liquid chromatography
Solvent extraction
Liquid Chromatography
Liver
Fish
Mass spectrometry
Mass Spectrometry
Fishes
Liver Extracts
Signal-To-Noise Ratio
Calibration
Limit of Detection
Linear Models
Linear regression
Regression analysis
Fats
Regression Analysis
Signal to noise ratio
Screening
bisphenol A

Keywords

  • Accelerated solvent extraction
  • Column liquid chromatography - mass spectrometry
  • Fish liver samples
  • Phenolic onti-oxidonts

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

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title = "Accelerated solvent extraction then liquid chromatography coupled with mass spectrometry for determination of 4-t-octylphenol, 4-nonylphenols, and bisphenol A in fish liver",
abstract = "Analysis of extracts from fish liver containing alkylphenol contaminants can be hindered by the presence of co-extracted fats ond proteins that interfere with chromatographic onalysis. In this study accelerated solvent extroction (ASE), Florisil clean-up, then combined liquid chromatography - mass spectrometry (LC-MS) with an electrospray (ESI) interface have been used to optimize an analytical procedure for the analysis of octylphenol, nonylphenol, and bisphenol A in fish liver. After comparison of the efficiency of ASE with conventionol Soxhlet extraction the developed procedure was applied to the analysis of liver samples. Calibration plots of the relationship between concentration and the ratio of the responses to the analyte and to the internol standard, 4-n-nonylphenol, were determined by linear regression analysis over the concentration range 0.05 to 10 ppm and resulted in good fits (r2 > 0.994). Recoveries (evaluated for each liver somple as the ratio between response to the surrogote compound, 4-n-nonylphenol, and that to the internal standard, 4-n-heptylphenol, relative to the some ratio for o reference standard solution) were 53 ± 20{\%}. Under the experimentol conditions used in this work the limits of detection (LOD), calculated by use of a signal-to-noise ratio of 3: 1, were 5 ng g-1 for 4-t-octylphenol, 15 ng g-1 for bisphenol A, and 20 ng g-1 for nonylphenol. The method can be sotisfactorily applied to screening analysis of octyl- and nonylphenols and bisphenol A in biological samples such as fish liver.",
keywords = "Accelerated solvent extraction, Column liquid chromatography - mass spectrometry, Fish liver samples, Phenolic onti-oxidonts",
author = "S. Tavazzi and E. Benfenati and D. Barcel{\'o}",
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T1 - Accelerated solvent extraction then liquid chromatography coupled with mass spectrometry for determination of 4-t-octylphenol, 4-nonylphenols, and bisphenol A in fish liver

AU - Tavazzi, S.

AU - Benfenati, E.

AU - Barceló, D.

PY - 2002/10

Y1 - 2002/10

N2 - Analysis of extracts from fish liver containing alkylphenol contaminants can be hindered by the presence of co-extracted fats ond proteins that interfere with chromatographic onalysis. In this study accelerated solvent extroction (ASE), Florisil clean-up, then combined liquid chromatography - mass spectrometry (LC-MS) with an electrospray (ESI) interface have been used to optimize an analytical procedure for the analysis of octylphenol, nonylphenol, and bisphenol A in fish liver. After comparison of the efficiency of ASE with conventionol Soxhlet extraction the developed procedure was applied to the analysis of liver samples. Calibration plots of the relationship between concentration and the ratio of the responses to the analyte and to the internol standard, 4-n-nonylphenol, were determined by linear regression analysis over the concentration range 0.05 to 10 ppm and resulted in good fits (r2 > 0.994). Recoveries (evaluated for each liver somple as the ratio between response to the surrogote compound, 4-n-nonylphenol, and that to the internal standard, 4-n-heptylphenol, relative to the some ratio for o reference standard solution) were 53 ± 20%. Under the experimentol conditions used in this work the limits of detection (LOD), calculated by use of a signal-to-noise ratio of 3: 1, were 5 ng g-1 for 4-t-octylphenol, 15 ng g-1 for bisphenol A, and 20 ng g-1 for nonylphenol. The method can be sotisfactorily applied to screening analysis of octyl- and nonylphenols and bisphenol A in biological samples such as fish liver.

AB - Analysis of extracts from fish liver containing alkylphenol contaminants can be hindered by the presence of co-extracted fats ond proteins that interfere with chromatographic onalysis. In this study accelerated solvent extroction (ASE), Florisil clean-up, then combined liquid chromatography - mass spectrometry (LC-MS) with an electrospray (ESI) interface have been used to optimize an analytical procedure for the analysis of octylphenol, nonylphenol, and bisphenol A in fish liver. After comparison of the efficiency of ASE with conventionol Soxhlet extraction the developed procedure was applied to the analysis of liver samples. Calibration plots of the relationship between concentration and the ratio of the responses to the analyte and to the internol standard, 4-n-nonylphenol, were determined by linear regression analysis over the concentration range 0.05 to 10 ppm and resulted in good fits (r2 > 0.994). Recoveries (evaluated for each liver somple as the ratio between response to the surrogote compound, 4-n-nonylphenol, and that to the internal standard, 4-n-heptylphenol, relative to the some ratio for o reference standard solution) were 53 ± 20%. Under the experimentol conditions used in this work the limits of detection (LOD), calculated by use of a signal-to-noise ratio of 3: 1, were 5 ng g-1 for 4-t-octylphenol, 15 ng g-1 for bisphenol A, and 20 ng g-1 for nonylphenol. The method can be sotisfactorily applied to screening analysis of octyl- and nonylphenols and bisphenol A in biological samples such as fish liver.

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KW - Phenolic onti-oxidonts

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