ACE gene polymorphism and insulin action in older subjects and healthy centenarians

Giuseppe Paolisso, Maria Rosaria Tagliamonte, Domenico De Lucia, Francesco Palmieri, Daniela Manzella, Carmela Rinaldi, Anna Bossone, Donatella Colaizzo, Maurizio Margaglione, Michele Varricchio

Research output: Contribution to journalArticlepeer-review


OBJECTIVES: To evaluate the possible relationship between angiotensin-converting enzyme (ACE) insertion-deletion (ID) genotype and insulin resistance in a population of healthy older Italian subjects. DESIGN: Prospective recruitment of a convenience sample. SETTING: Outpatient clinic. PARTICIPANTS: One hundred twenty-five subjects age 62 to 105 in good health and not taking any drug known to interfere with glucose metabolism. MEASUREMENTS: Anthropometric measurements; fasting plasma glucose (FPG), and fasting plasma insulin (FPI) concentrations; oral glucose tolerance test; homeostatic method assessment (HOMA) to estimate degree of insulin resistance; and ACE genotype by polymerase chain reaction. RESULTS: In the sample population, the relative frequencies of the ACE genotypes deletion-deletion (DD) (0.424), ID (0.400), and insertion-insertion (II) (0.176) were not significantly different from values predicted by Hardy-Weinberg equilibrium. The genotype distribution was similar in men and women. Subjects carrying the II genotype had a higher FPG (P <.001) and FPI (P <.001) than did subjects with DD or ID genotype. Subjects with II genotype also had a significantly higher HOMA index than did subjects with DD or ID genotype (P for trend <.002). In a multivariate stepwise regression analysis, the ACE ID polymorphism was significantly and independently associated with the HOMA index (P <.001). The same result was confirmed performing multivariate analysis in the younger group and centenarians separately. CONCLUSION: In an older population, the presence of II ACE genotype is associated with a high degree of insulin resistance independent of other anthropometric variables known to interfere with insulin action; this association is significant in both the younger subjects and the centenarians.

Original languageEnglish
Pages (from-to)610-614
Number of pages5
JournalJournal of the American Geriatrics Society
Issue number5
Publication statusPublished - 2001


  • Angiotensin-converting enzyme
  • Centenarians
  • Genotype
  • Insulin resistance
  • Older population

ASJC Scopus subject areas

  • Geriatrics and Gerontology


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