Abstract
OBJECTIVES: To evaluate the possible relationship between angiotensin-converting enzyme (ACE) insertion-deletion (ID) genotype and insulin resistance in a population of healthy older Italian subjects. DESIGN: Prospective recruitment of a convenience sample. SETTING: Outpatient clinic. PARTICIPANTS: One hundred twenty-five subjects age 62 to 105 in good health and not taking any drug known to interfere with glucose metabolism. MEASUREMENTS: Anthropometric measurements; fasting plasma glucose (FPG), and fasting plasma insulin (FPI) concentrations; oral glucose tolerance test; homeostatic method assessment (HOMA) to estimate degree of insulin resistance; and ACE genotype by polymerase chain reaction. RESULTS: In the sample population, the relative frequencies of the ACE genotypes deletion-deletion (DD) (0.424), ID (0.400), and insertion-insertion (II) (0.176) were not significantly different from values predicted by Hardy-Weinberg equilibrium. The genotype distribution was similar in men and women. Subjects carrying the II genotype had a higher FPG (P <.001) and FPI (P <.001) than did subjects with DD or ID genotype. Subjects with II genotype also had a significantly higher HOMA index than did subjects with DD or ID genotype (P for trend <.002). In a multivariate stepwise regression analysis, the ACE ID polymorphism was significantly and independently associated with the HOMA index (P <.001). The same result was confirmed performing multivariate analysis in the younger group and centenarians separately. CONCLUSION: In an older population, the presence of II ACE genotype is associated with a high degree of insulin resistance independent of other anthropometric variables known to interfere with insulin action; this association is significant in both the younger subjects and the centenarians.
| Original language | English |
|---|---|
| Pages (from-to) | 610-614 |
| Number of pages | 5 |
| Journal | Journal of the American Geriatrics Society |
| Volume | 49 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2001 |
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Keywords
- Angiotensin-converting enzyme
- Centenarians
- Genotype
- Insulin resistance
- Older population
ASJC Scopus subject areas
- Geriatrics and Gerontology
Cite this
ACE gene polymorphism and insulin action in older subjects and healthy centenarians. / Paolisso, Giuseppe; Tagliamonte, Maria Rosaria; De Lucia, Domenico; Palmieri, Francesco; Manzella, Daniela; Rinaldi, Carmela; Bossone, Anna; Colaizzo, Donatella; Margaglione, Maurizio; Varricchio, Michele.
In: Journal of the American Geriatrics Society, Vol. 49, No. 5, 2001, p. 610-614.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - ACE gene polymorphism and insulin action in older subjects and healthy centenarians
AU - Paolisso, Giuseppe
AU - Tagliamonte, Maria Rosaria
AU - De Lucia, Domenico
AU - Palmieri, Francesco
AU - Manzella, Daniela
AU - Rinaldi, Carmela
AU - Bossone, Anna
AU - Colaizzo, Donatella
AU - Margaglione, Maurizio
AU - Varricchio, Michele
PY - 2001
Y1 - 2001
N2 - OBJECTIVES: To evaluate the possible relationship between angiotensin-converting enzyme (ACE) insertion-deletion (ID) genotype and insulin resistance in a population of healthy older Italian subjects. DESIGN: Prospective recruitment of a convenience sample. SETTING: Outpatient clinic. PARTICIPANTS: One hundred twenty-five subjects age 62 to 105 in good health and not taking any drug known to interfere with glucose metabolism. MEASUREMENTS: Anthropometric measurements; fasting plasma glucose (FPG), and fasting plasma insulin (FPI) concentrations; oral glucose tolerance test; homeostatic method assessment (HOMA) to estimate degree of insulin resistance; and ACE genotype by polymerase chain reaction. RESULTS: In the sample population, the relative frequencies of the ACE genotypes deletion-deletion (DD) (0.424), ID (0.400), and insertion-insertion (II) (0.176) were not significantly different from values predicted by Hardy-Weinberg equilibrium. The genotype distribution was similar in men and women. Subjects carrying the II genotype had a higher FPG (P <.001) and FPI (P <.001) than did subjects with DD or ID genotype. Subjects with II genotype also had a significantly higher HOMA index than did subjects with DD or ID genotype (P for trend <.002). In a multivariate stepwise regression analysis, the ACE ID polymorphism was significantly and independently associated with the HOMA index (P <.001). The same result was confirmed performing multivariate analysis in the younger group and centenarians separately. CONCLUSION: In an older population, the presence of II ACE genotype is associated with a high degree of insulin resistance independent of other anthropometric variables known to interfere with insulin action; this association is significant in both the younger subjects and the centenarians.
AB - OBJECTIVES: To evaluate the possible relationship between angiotensin-converting enzyme (ACE) insertion-deletion (ID) genotype and insulin resistance in a population of healthy older Italian subjects. DESIGN: Prospective recruitment of a convenience sample. SETTING: Outpatient clinic. PARTICIPANTS: One hundred twenty-five subjects age 62 to 105 in good health and not taking any drug known to interfere with glucose metabolism. MEASUREMENTS: Anthropometric measurements; fasting plasma glucose (FPG), and fasting plasma insulin (FPI) concentrations; oral glucose tolerance test; homeostatic method assessment (HOMA) to estimate degree of insulin resistance; and ACE genotype by polymerase chain reaction. RESULTS: In the sample population, the relative frequencies of the ACE genotypes deletion-deletion (DD) (0.424), ID (0.400), and insertion-insertion (II) (0.176) were not significantly different from values predicted by Hardy-Weinberg equilibrium. The genotype distribution was similar in men and women. Subjects carrying the II genotype had a higher FPG (P <.001) and FPI (P <.001) than did subjects with DD or ID genotype. Subjects with II genotype also had a significantly higher HOMA index than did subjects with DD or ID genotype (P for trend <.002). In a multivariate stepwise regression analysis, the ACE ID polymorphism was significantly and independently associated with the HOMA index (P <.001). The same result was confirmed performing multivariate analysis in the younger group and centenarians separately. CONCLUSION: In an older population, the presence of II ACE genotype is associated with a high degree of insulin resistance independent of other anthropometric variables known to interfere with insulin action; this association is significant in both the younger subjects and the centenarians.
KW - Angiotensin-converting enzyme
KW - Centenarians
KW - Genotype
KW - Insulin resistance
KW - Older population
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UR - http://www.scopus.com/inward/citedby.url?scp=0035009651&partnerID=8YFLogxK
U2 - 10.1046/j.1532-5415.2001.49122.x
DO - 10.1046/j.1532-5415.2001.49122.x
M3 - Article
VL - 49
SP - 610
EP - 614
JO - Journal of the American Geriatrics Society
JF - Journal of the American Geriatrics Society
SN - 0002-8614
IS - 5
ER -