Acetylcholine-induced production of platelet-activating factor by human fetal brain cells in culture

V. Sogos, F. Bussolino, E. Pilia, S. Torelli, F. Gremo

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Platelet-activating-factor (PAF) is a potent, biologically active lipid mediator produced by several tissues, including brain. Its role in the central nervous system (CNS) is still unknown, even if its involvement in brain damage and neurotoxicity has been postulated. Its production by neural cells has been demonstrated in different species, but not in man. This paper provides evidence that PAF can be produced by human fetal neurons and/or glial cells in culture. Its synthesis dramatically increased upon stimulation with acetylcholine (ACh), and it was significantly lowered by the cholinergic receptor antagonist atropine. Almost no PAF was detected in the incubation medium, which indicated no release of PAF from cultured cells. Characterization of the cells in culture with specific monoclonal antibodies excluded the presence of endothelial cells or macrophages, which also produce PAF.

Original languageEnglish
Pages (from-to)706-711
Number of pages6
JournalJournal of Neuroscience Research
Volume27
Issue number4
DOIs
Publication statusPublished - 1990

Fingerprint

Platelet Activating Factor
Acetylcholine
Cell Culture Techniques
Brain
Cholinergic Antagonists
Atropine
Neuroglia
Cultured Cells
Central Nervous System
Endothelial Cells
Macrophages
Monoclonal Antibodies
Lipids
Neurons

Keywords

  • acetylcholine
  • cultures
  • human fetal brain
  • platelet-activating factor (PAF)

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Acetylcholine-induced production of platelet-activating factor by human fetal brain cells in culture. / Sogos, V.; Bussolino, F.; Pilia, E.; Torelli, S.; Gremo, F.

In: Journal of Neuroscience Research, Vol. 27, No. 4, 1990, p. 706-711.

Research output: Contribution to journalArticle

@article{dcce8f5574c64147b2a93eb38dc63d02,
title = "Acetylcholine-induced production of platelet-activating factor by human fetal brain cells in culture",
abstract = "Platelet-activating-factor (PAF) is a potent, biologically active lipid mediator produced by several tissues, including brain. Its role in the central nervous system (CNS) is still unknown, even if its involvement in brain damage and neurotoxicity has been postulated. Its production by neural cells has been demonstrated in different species, but not in man. This paper provides evidence that PAF can be produced by human fetal neurons and/or glial cells in culture. Its synthesis dramatically increased upon stimulation with acetylcholine (ACh), and it was significantly lowered by the cholinergic receptor antagonist atropine. Almost no PAF was detected in the incubation medium, which indicated no release of PAF from cultured cells. Characterization of the cells in culture with specific monoclonal antibodies excluded the presence of endothelial cells or macrophages, which also produce PAF.",
keywords = "acetylcholine, cultures, human fetal brain, platelet-activating factor (PAF)",
author = "V. Sogos and F. Bussolino and E. Pilia and S. Torelli and F. Gremo",
year = "1990",
doi = "10.1002/jnr.490270431",
language = "English",
volume = "27",
pages = "706--711",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Acetylcholine-induced production of platelet-activating factor by human fetal brain cells in culture

AU - Sogos, V.

AU - Bussolino, F.

AU - Pilia, E.

AU - Torelli, S.

AU - Gremo, F.

PY - 1990

Y1 - 1990

N2 - Platelet-activating-factor (PAF) is a potent, biologically active lipid mediator produced by several tissues, including brain. Its role in the central nervous system (CNS) is still unknown, even if its involvement in brain damage and neurotoxicity has been postulated. Its production by neural cells has been demonstrated in different species, but not in man. This paper provides evidence that PAF can be produced by human fetal neurons and/or glial cells in culture. Its synthesis dramatically increased upon stimulation with acetylcholine (ACh), and it was significantly lowered by the cholinergic receptor antagonist atropine. Almost no PAF was detected in the incubation medium, which indicated no release of PAF from cultured cells. Characterization of the cells in culture with specific monoclonal antibodies excluded the presence of endothelial cells or macrophages, which also produce PAF.

AB - Platelet-activating-factor (PAF) is a potent, biologically active lipid mediator produced by several tissues, including brain. Its role in the central nervous system (CNS) is still unknown, even if its involvement in brain damage and neurotoxicity has been postulated. Its production by neural cells has been demonstrated in different species, but not in man. This paper provides evidence that PAF can be produced by human fetal neurons and/or glial cells in culture. Its synthesis dramatically increased upon stimulation with acetylcholine (ACh), and it was significantly lowered by the cholinergic receptor antagonist atropine. Almost no PAF was detected in the incubation medium, which indicated no release of PAF from cultured cells. Characterization of the cells in culture with specific monoclonal antibodies excluded the presence of endothelial cells or macrophages, which also produce PAF.

KW - acetylcholine

KW - cultures

KW - human fetal brain

KW - platelet-activating factor (PAF)

UR - http://www.scopus.com/inward/record.url?scp=0025641181&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025641181&partnerID=8YFLogxK

U2 - 10.1002/jnr.490270431

DO - 10.1002/jnr.490270431

M3 - Article

VL - 27

SP - 706

EP - 711

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 4

ER -