Abstract
Platelet-activating-factor (PAF) is a potent, biologically active lipid mediator produced by several tissues, including brain. Its role in the central nervous system (CNS) is still unknown, even if its involvement in brain damage and neurotoxicity has been postulated. Its production by neural cells has been demonstrated in different species, but not in man. This paper provides evidence that PAF can be produced by human fetal neurons and/or glial cells in culture. Its synthesis dramatically increased upon stimulation with acetylcholine (ACh), and it was significantly lowered by the cholinergic receptor antagonist atropine. Almost no PAF was detected in the incubation medium, which indicated no release of PAF from cultured cells. Characterization of the cells in culture with specific monoclonal antibodies excluded the presence of endothelial cells or macrophages, which also produce PAF.
| Original language | English |
|---|---|
| Pages (from-to) | 706-711 |
| Number of pages | 6 |
| Journal | Journal of Neuroscience Research |
| Volume | 27 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1990 |
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Keywords
- acetylcholine
- cultures
- human fetal brain
- platelet-activating factor (PAF)
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Acetylcholine-induced production of platelet-activating factor by human fetal brain cells in culture. / Sogos, V.; Bussolino, F.; Pilia, E.; Torelli, S.; Gremo, F.
In: Journal of Neuroscience Research, Vol. 27, No. 4, 1990, p. 706-711.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Acetylcholine-induced production of platelet-activating factor by human fetal brain cells in culture
AU - Sogos, V.
AU - Bussolino, F.
AU - Pilia, E.
AU - Torelli, S.
AU - Gremo, F.
PY - 1990
Y1 - 1990
N2 - Platelet-activating-factor (PAF) is a potent, biologically active lipid mediator produced by several tissues, including brain. Its role in the central nervous system (CNS) is still unknown, even if its involvement in brain damage and neurotoxicity has been postulated. Its production by neural cells has been demonstrated in different species, but not in man. This paper provides evidence that PAF can be produced by human fetal neurons and/or glial cells in culture. Its synthesis dramatically increased upon stimulation with acetylcholine (ACh), and it was significantly lowered by the cholinergic receptor antagonist atropine. Almost no PAF was detected in the incubation medium, which indicated no release of PAF from cultured cells. Characterization of the cells in culture with specific monoclonal antibodies excluded the presence of endothelial cells or macrophages, which also produce PAF.
AB - Platelet-activating-factor (PAF) is a potent, biologically active lipid mediator produced by several tissues, including brain. Its role in the central nervous system (CNS) is still unknown, even if its involvement in brain damage and neurotoxicity has been postulated. Its production by neural cells has been demonstrated in different species, but not in man. This paper provides evidence that PAF can be produced by human fetal neurons and/or glial cells in culture. Its synthesis dramatically increased upon stimulation with acetylcholine (ACh), and it was significantly lowered by the cholinergic receptor antagonist atropine. Almost no PAF was detected in the incubation medium, which indicated no release of PAF from cultured cells. Characterization of the cells in culture with specific monoclonal antibodies excluded the presence of endothelial cells or macrophages, which also produce PAF.
KW - acetylcholine
KW - cultures
KW - human fetal brain
KW - platelet-activating factor (PAF)
UR - http://www.scopus.com/inward/record.url?scp=0025641181&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025641181&partnerID=8YFLogxK
U2 - 10.1002/jnr.490270431
DO - 10.1002/jnr.490270431
M3 - Article
C2 - 2079725
AN - SCOPUS:0025641181
VL - 27
SP - 706
EP - 711
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 4
ER -