Acetylcholine induces voltage-independent increase of cytosolic calcium in mouse myotubes

Aldo Giovanelli, Francesca Grassi, Elisabetta Mattei, Anna M. Mileo, Fabrizio Eusebi

Research output: Contribution to journalArticlepeer-review


Electrophysiological, biochemical, and Ca2+ imaging studies of cultured mouse myotubes were used to investigate whether the neurotransmitter acetylcholine causes an increase in intracellular Ca2+ concentration ([Ca2+]i through activation of a second messenger system. Bath applications of acetylcholine to myotubes (i) elicited a significant membrane current even in a Na+-free Ca2+ medium, when the current was carried mainly by calcium ions; (ii) caused a rapid and transient cytosolic accumulation of inositol 1,4,5-triphosphate; (iii) evoked a conspicuous α-bungarotoxin-sensitive long-lasting [Ca2+]i enhancement even in the presence of Cd2+; and (iv) transiently increased [Ca2+]i when cells were equilibrated in a Ca2+-free atropine-containmg medium. We propose that, in addition to opening ion channels, the nicotinic action of acetylcholine on the muscle cell membrane increases [Ca2+]i through activation of the inositol 1,4,5-trisphosphate second messenger system and mobilization of Ca2+ from intracellular stores. (.

Original languageEnglish
Pages (from-to)10069-10073
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number22
Publication statusPublished - 1991


  • Acetylchollne receptors
  • Ca transients
  • Inositol 1,4,5-trisphosphate

ASJC Scopus subject areas

  • General
  • Genetics


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