TY - JOUR
T1 - Acid citrate dextrose (ACD) formula A as a new anticoagulant in the measurement of in vitro platelet aggregation
AU - Pignatelli, P.
AU - Pulcinelli, F. M.
AU - Ciatti, F.
AU - Pesciotti, M.
AU - Sebastiani, S.
AU - Ferroni, P.
AU - Gazzaniga, P. P.
PY - 1995
Y1 - 1995
N2 - To evaluate whether the use of ACD Formula A may affect in vitro platelet function, blood samples were obtained from 21 healthy blood donors and anticoagulated in ACD (acid-citrate dextrose, NIH Formula A), Na citrate 3.8%, and K3EDTA. Platelet count, mean platelet volume, and in vitro platelet aggregation were evaluated on each sample. No significant difference was observed in platelet count and mean platelet volume among the different samples. Conversely, the ACD treated platelets showed a higher reactivity to the agonists as demonstrated by a significant increase of the maximum percentages of aggregation induced by ADP, epinephrine, and collagen, as well as a significant decrease of secondary aggregation thresholds to ADP and epinephrine. In conclusion, it may be speculated that ACD Formula A is capable of better maintaining the intraplatelet signal transduction mechanisms during PRP preparation, thus improving the overall responsiveness of platelets.
AB - To evaluate whether the use of ACD Formula A may affect in vitro platelet function, blood samples were obtained from 21 healthy blood donors and anticoagulated in ACD (acid-citrate dextrose, NIH Formula A), Na citrate 3.8%, and K3EDTA. Platelet count, mean platelet volume, and in vitro platelet aggregation were evaluated on each sample. No significant difference was observed in platelet count and mean platelet volume among the different samples. Conversely, the ACD treated platelets showed a higher reactivity to the agonists as demonstrated by a significant increase of the maximum percentages of aggregation induced by ADP, epinephrine, and collagen, as well as a significant decrease of secondary aggregation thresholds to ADP and epinephrine. In conclusion, it may be speculated that ACD Formula A is capable of better maintaining the intraplatelet signal transduction mechanisms during PRP preparation, thus improving the overall responsiveness of platelets.
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U2 - 10.1002/jcla.1860090211
DO - 10.1002/jcla.1860090211
M3 - Article
C2 - 7714666
AN - SCOPUS:0028878204
VL - 9
SP - 138
EP - 140
JO - Journal of Clinical Laboratory Analysis
JF - Journal of Clinical Laboratory Analysis
SN - 0887-8013
IS - 2
ER -