Acid lability is not an intrinsic property of interferon-α induced by HIV-infected cells

M. R. Capobianchi, P. Mattana, F. Mercuri, G. Conciatori, F. Ameglio, H. Ankel, F. Dianzani

Research output: Contribution to journalArticle

Abstract

Human immunodeficiency virus (HIV)-infected cells induce acid-labile interferon-α (al-IFN-α) in cultures of mononuclear cells from peripheral human blood. We have investigated the physicochemical properties of such preparations to elucidate the reasons for acid-lability of this IFN. Al-IFN- α is a mixture of both glycosylated and unglycosylated molecules as shown by separation on Concanavalin-A Sepharose. Acid-lability is associated only with glycosylated molecules. Upon chromatography of the glycosylated fraction on Sepharose coupled to IFN-α-specific antibody, the portion of the IFN that is retained and eluted with guanidine-HCl is acid-stable, whereas the excluded antiviral activity is acid-labile, and is partially neutralized by antibodies to either IFN-α or IFN-γ. Also, upon further purification of the unglycosylated fraction on the same antibody column, all antiviral activity remains indistinguishable from conventional IFN-α. Reconstitution experiments showed that glycosylated material excluded from the anti-IFN-α column potentiates antiviral activity of the IFN that is specifically retained by the column. This potentiation is abolished by acid treatment. Similar results are obtained with al-IFN-α from the serum of acquired immunodeficiency syndrome (AIDS) patients, indicating that its acid-lability is also the consequence of an acid-labile component that is capable of enhancing the antiviral activity. The potentiation of antiviral activity obtained by combining recombinant preparations of IFN-α and IFN-γ suggests that the cooperating molecule is IFN-γ. The presence of IFN-γ in al-IFN-α as determined by ELISA assay, by affinity chromatography on a column containing anti-IFN-γ antibody, and by dot-blot analysis of its mRNA in the induced cells, supports our conclusion that (i) there is no acid-labile IFN- α per se, (ii) al-IFN-α is a mixture of conventional IFN-α and IFN-γ, and (iii) the apparent acid-lability of al-IFN-α is due to the synergistic antiviral effects of acid-stable IFN-α and acid-labile IFN-γ which are both present in al-IFN-α.

Original languageEnglish
Pages (from-to)431-438
Number of pages8
JournalJournal of Interferon Research
Volume12
Issue number6
Publication statusPublished - 1992

Fingerprint

Interferons
HIV
Acids
Antiviral Agents
Antibodies
Guanidine
Affinity Chromatography
Sepharose
Chromatography
Anti-Idiotypic Antibodies
Acquired Immunodeficiency Syndrome

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Capobianchi, M. R., Mattana, P., Mercuri, F., Conciatori, G., Ameglio, F., Ankel, H., & Dianzani, F. (1992). Acid lability is not an intrinsic property of interferon-α induced by HIV-infected cells. Journal of Interferon Research, 12(6), 431-438.

Acid lability is not an intrinsic property of interferon-α induced by HIV-infected cells. / Capobianchi, M. R.; Mattana, P.; Mercuri, F.; Conciatori, G.; Ameglio, F.; Ankel, H.; Dianzani, F.

In: Journal of Interferon Research, Vol. 12, No. 6, 1992, p. 431-438.

Research output: Contribution to journalArticle

Capobianchi, MR, Mattana, P, Mercuri, F, Conciatori, G, Ameglio, F, Ankel, H & Dianzani, F 1992, 'Acid lability is not an intrinsic property of interferon-α induced by HIV-infected cells', Journal of Interferon Research, vol. 12, no. 6, pp. 431-438.
Capobianchi, M. R. ; Mattana, P. ; Mercuri, F. ; Conciatori, G. ; Ameglio, F. ; Ankel, H. ; Dianzani, F. / Acid lability is not an intrinsic property of interferon-α induced by HIV-infected cells. In: Journal of Interferon Research. 1992 ; Vol. 12, No. 6. pp. 431-438.
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AU - Mattana, P.

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AU - Ankel, H.

AU - Dianzani, F.

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