Acid -sensing ion channel 1a is required for mGlu receptor dependent long-term depression in the hippocampus

D. Mango, E. Braksator, G. Battaglia, S. Marcelli, N. B. Mercuri, M. Feligioni, F. Nicoletti, Z. I. Bashir, R. Nistico

Research output: Contribution to journalArticle

Abstract

Acid-sensing ion channels (ASICs), members of the degenerin/epithelial Na+ channel superfamily, are widely distributed in the mammalian nervous system. ASIC1 a is highly permeable to Ca2+ and are thought to be important in a variety of physiological processes, including synaptic plasticity, learning and memory. To further understand the role of ASIC1a in synaptic transmission and plasticity, we investigated metabotropic glutamate (mGlu) receptor-dependent long-term depression (LTD) in the hippocampus. We found that ASIC1a channels mediate a component of LTD in P30-40 animals, since the ASIC1a selective blocker psalmotoxin-1 (PcTx1) reduced the magnitude of LTD induced by application of the group I mGlu receptor agonist (S)-3,5-Dihydroxyphenylglycine (DHPG) or induced by paired-pulse low frequency stimulation (PP-LFS). Conversely, PcTx1 did not affect LTD in P13-18 animals. We also provide evidence that ASIC1a is involved in group I mGlu receptor-induced increase in action potential firing. However, blockade of ASIC1a did not affect DHPG-induced polyphosphoinositide hydrolysis, suggesting the involvement of some other molecular partners in the functional crosstalk between ASIC1a and group I mGlu receptors. Notably, PcTx1 was able to prevent the increase in GluA1 5845 phosphorylation at the post-synaptic membrane induced by group I mGlu receptor activation. These findings suggest a novel function of ASIC1 a channels in the regulation of group I mGlu receptor synaptic plasticity and intrinsic excitability. (C) 2017 Elsevier Ltd. All rights reserved.
Original languageUndefined/Unknown
Pages (from-to)12-19
Number of pages8
JournalPharmacological Research
Volume119
DOIs
Publication statusPublished - Mar 1 2017

Keywords

  • ASIC
  • Hippocampus
  • Electrophysiology
  • LTD
  • mGlu receptors

Cite this

Acid -sensing ion channel 1a is required for mGlu receptor dependent long-term depression in the hippocampus. / Mango, D.; Braksator, E.; Battaglia, G.; Marcelli, S.; Mercuri, N. B.; Feligioni, M.; Nicoletti, F.; Bashir, Z. I.; Nistico, R.

In: Pharmacological Research, Vol. 119, 01.03.2017, p. 12-19.

Research output: Contribution to journalArticle

Mango, D. ; Braksator, E. ; Battaglia, G. ; Marcelli, S. ; Mercuri, N. B. ; Feligioni, M. ; Nicoletti, F. ; Bashir, Z. I. ; Nistico, R. / Acid -sensing ion channel 1a is required for mGlu receptor dependent long-term depression in the hippocampus. In: Pharmacological Research. 2017 ; Vol. 119. pp. 12-19.
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abstract = "Acid-sensing ion channels (ASICs), members of the degenerin/epithelial Na+ channel superfamily, are widely distributed in the mammalian nervous system. ASIC1 a is highly permeable to Ca2+ and are thought to be important in a variety of physiological processes, including synaptic plasticity, learning and memory. To further understand the role of ASIC1a in synaptic transmission and plasticity, we investigated metabotropic glutamate (mGlu) receptor-dependent long-term depression (LTD) in the hippocampus. We found that ASIC1a channels mediate a component of LTD in P30-40 animals, since the ASIC1a selective blocker psalmotoxin-1 (PcTx1) reduced the magnitude of LTD induced by application of the group I mGlu receptor agonist (S)-3,5-Dihydroxyphenylglycine (DHPG) or induced by paired-pulse low frequency stimulation (PP-LFS). Conversely, PcTx1 did not affect LTD in P13-18 animals. We also provide evidence that ASIC1a is involved in group I mGlu receptor-induced increase in action potential firing. However, blockade of ASIC1a did not affect DHPG-induced polyphosphoinositide hydrolysis, suggesting the involvement of some other molecular partners in the functional crosstalk between ASIC1a and group I mGlu receptors. Notably, PcTx1 was able to prevent the increase in GluA1 5845 phosphorylation at the post-synaptic membrane induced by group I mGlu receptor activation. These findings suggest a novel function of ASIC1 a channels in the regulation of group I mGlu receptor synaptic plasticity and intrinsic excitability. (C) 2017 Elsevier Ltd. All rights reserved.",
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T1 - Acid -sensing ion channel 1a is required for mGlu receptor dependent long-term depression in the hippocampus

AU - Mango, D.

AU - Braksator, E.

AU - Battaglia, G.

AU - Marcelli, S.

AU - Mercuri, N. B.

AU - Feligioni, M.

AU - Nicoletti, F.

AU - Bashir, Z. I.

AU - Nistico, R.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Acid-sensing ion channels (ASICs), members of the degenerin/epithelial Na+ channel superfamily, are widely distributed in the mammalian nervous system. ASIC1 a is highly permeable to Ca2+ and are thought to be important in a variety of physiological processes, including synaptic plasticity, learning and memory. To further understand the role of ASIC1a in synaptic transmission and plasticity, we investigated metabotropic glutamate (mGlu) receptor-dependent long-term depression (LTD) in the hippocampus. We found that ASIC1a channels mediate a component of LTD in P30-40 animals, since the ASIC1a selective blocker psalmotoxin-1 (PcTx1) reduced the magnitude of LTD induced by application of the group I mGlu receptor agonist (S)-3,5-Dihydroxyphenylglycine (DHPG) or induced by paired-pulse low frequency stimulation (PP-LFS). Conversely, PcTx1 did not affect LTD in P13-18 animals. We also provide evidence that ASIC1a is involved in group I mGlu receptor-induced increase in action potential firing. However, blockade of ASIC1a did not affect DHPG-induced polyphosphoinositide hydrolysis, suggesting the involvement of some other molecular partners in the functional crosstalk between ASIC1a and group I mGlu receptors. Notably, PcTx1 was able to prevent the increase in GluA1 5845 phosphorylation at the post-synaptic membrane induced by group I mGlu receptor activation. These findings suggest a novel function of ASIC1 a channels in the regulation of group I mGlu receptor synaptic plasticity and intrinsic excitability. (C) 2017 Elsevier Ltd. All rights reserved.

AB - Acid-sensing ion channels (ASICs), members of the degenerin/epithelial Na+ channel superfamily, are widely distributed in the mammalian nervous system. ASIC1 a is highly permeable to Ca2+ and are thought to be important in a variety of physiological processes, including synaptic plasticity, learning and memory. To further understand the role of ASIC1a in synaptic transmission and plasticity, we investigated metabotropic glutamate (mGlu) receptor-dependent long-term depression (LTD) in the hippocampus. We found that ASIC1a channels mediate a component of LTD in P30-40 animals, since the ASIC1a selective blocker psalmotoxin-1 (PcTx1) reduced the magnitude of LTD induced by application of the group I mGlu receptor agonist (S)-3,5-Dihydroxyphenylglycine (DHPG) or induced by paired-pulse low frequency stimulation (PP-LFS). Conversely, PcTx1 did not affect LTD in P13-18 animals. We also provide evidence that ASIC1a is involved in group I mGlu receptor-induced increase in action potential firing. However, blockade of ASIC1a did not affect DHPG-induced polyphosphoinositide hydrolysis, suggesting the involvement of some other molecular partners in the functional crosstalk between ASIC1a and group I mGlu receptors. Notably, PcTx1 was able to prevent the increase in GluA1 5845 phosphorylation at the post-synaptic membrane induced by group I mGlu receptor activation. These findings suggest a novel function of ASIC1 a channels in the regulation of group I mGlu receptor synaptic plasticity and intrinsic excitability. (C) 2017 Elsevier Ltd. All rights reserved.

KW - ASIC

KW - Hippocampus

KW - Electrophysiology

KW - LTD

KW - mGlu receptors

U2 - 10.1016/j.phrs.2017.01.028

DO - 10.1016/j.phrs.2017.01.028

M3 - Articolo

VL - 119

SP - 12

EP - 19

JO - Pharmacological Research

JF - Pharmacological Research

SN - 1043-6618

ER -