Acid sensing ion channel 2: A new potential player in the pathophysiology of multiple sclerosis

Teresa Fazia, Roberta Pastorino, Serena Notartomaso, Carla Busceti, Tiziana Imbriglio, Milena Cannella, Davide Gentilini, Gabriele Morani, Anna Ticca, Pierpaolo Bitti, Carlo Berzuini, Tamas Dalmay, Giuseppe Battaglia, Luisa Bernardinelli

Research output: Contribution to journalArticle

Abstract

Acid-sensing ion channels (ASICs) are proton-gated channels involved in multiple biological functions such as: pain modulation, mechanosensation, neurotransmission, and neurodegeneration. Earlier, we described the genetic association, within the Nuoro population, between Multiple Sclerosis (MS) and rs28936, located in ASIC2 3′UTR. Here we investigated the potential involvement of ASIC2 in MS inflammatory process. We induced experimental autoimmune encephalomyelitis (EAE) in wild-type (WT), knockout Asic1−/− and Asic2−/− mice and observed a significant reduction of clinical score in Asic1−/− mice and a significant reduction in the clinical score in Asic2−/− mice in a limited time window (i.e., at days 20–23 after immunization). Immunohistochemistry confirmed the reduction in adaptive immune cell infiltrates in the spinal cord of EAE Asic1−/− mice. Analysis of mechanical allodynia, showed a significant higher pain threshold in Asic2−/− mice under physiological conditions, before immunization, as compared to WT mice and Asic1−/−. A significant reduction in pain threshold was observed in all three strains of mice after immunization. More importantly, analysis of human autoptic brain tissue in MS and control samples showed an increase of ASIC2 mRNA in MS samples. Subsequently, in vitro luciferase reporter gene assays, showed that ASIC2 expression is under possible miRNA regulation, in a rs28936 allele-specific manner. Taken together, these findings suggest a potential role of ASIC2 in the pathophysiology of MS.

Original languageEnglish
Pages (from-to)1233-1243
Number of pages11
JournalEuropean Journal of Neuroscience
Volume49
Issue number10
DOIs
Publication statusPublished - May 2019

Fingerprint

Acid Sensing Ion Channels
Multiple Sclerosis
Immunization
Pain Threshold
Autoimmune Experimental Encephalomyelitis
Hyperalgesia
Luciferases
MicroRNAs
Reporter Genes
Synaptic Transmission
Protons
Spinal Cord
Immunohistochemistry
Alleles
Pain
Messenger RNA
Brain

Keywords

  • ASIC1
  • ASIC2
  • experimental autoimmune encephalomyelitis
  • mechanosensation
  • mouse models

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Acid sensing ion channel 2 : A new potential player in the pathophysiology of multiple sclerosis. / Fazia, Teresa; Pastorino, Roberta; Notartomaso, Serena; Busceti, Carla; Imbriglio, Tiziana; Cannella, Milena; Gentilini, Davide; Morani, Gabriele; Ticca, Anna; Bitti, Pierpaolo; Berzuini, Carlo; Dalmay, Tamas; Battaglia, Giuseppe; Bernardinelli, Luisa.

In: European Journal of Neuroscience, Vol. 49, No. 10, 05.2019, p. 1233-1243.

Research output: Contribution to journalArticle

Fazia, T, Pastorino, R, Notartomaso, S, Busceti, C, Imbriglio, T, Cannella, M, Gentilini, D, Morani, G, Ticca, A, Bitti, P, Berzuini, C, Dalmay, T, Battaglia, G & Bernardinelli, L 2019, 'Acid sensing ion channel 2: A new potential player in the pathophysiology of multiple sclerosis', European Journal of Neuroscience, vol. 49, no. 10, pp. 1233-1243. https://doi.org/10.1111/ejn.14302
Fazia, Teresa ; Pastorino, Roberta ; Notartomaso, Serena ; Busceti, Carla ; Imbriglio, Tiziana ; Cannella, Milena ; Gentilini, Davide ; Morani, Gabriele ; Ticca, Anna ; Bitti, Pierpaolo ; Berzuini, Carlo ; Dalmay, Tamas ; Battaglia, Giuseppe ; Bernardinelli, Luisa. / Acid sensing ion channel 2 : A new potential player in the pathophysiology of multiple sclerosis. In: European Journal of Neuroscience. 2019 ; Vol. 49, No. 10. pp. 1233-1243.
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