Abstract
Malignant melanoma incidence is increasing rapidly in Western countries. Its prevention requires a deep knowledge of the biological basis of the neoplasm leading to the identification of new biological risk markers. In in-vitro and ex-vivo models we demonstrated that catalase was modified not only in its activity but also in its charge properties after ultraviolet A irradiation through pheomelanin. Here we focus on the electrophoretic behaviour of catalase in the human skin in vivo, in association with cutaneous phototype. Zymographic analysis of the enzyme on skin biopsies from Caucasian population (phototype I-IV), collected from the trunk in autumn-winter, to exclude possible influences of an acute photoexposure, evidenced a protein doublet, representing the coexistence of two active isoforms of catalase with different charge properties. In the skin from low-phototype subjects, the percent contribution of the more acidic component of the doublet was prevalent, inversely correlated with total melanin concentration in hair, and associated with a high number of melanocytic nevi. In summary, this study shows for the first time the existence of an acidic catalase in association with clinically defined risk characteristics in low phototype skin in vivo, contributing to the knowledge of a new biochemical marker of cutaneous photosusceptibility. Melanoma Res 19:372-378
| Original language | English |
|---|---|
| Pages (from-to) | 372-378 |
| Number of pages | 7 |
| Journal | Melanoma Research |
| Volume | 19 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2009 |
Fingerprint
Keywords
- Cutaneous phototype
- Hair melanin
- Native catalase
- Skin biopsies
ASJC Scopus subject areas
- Cancer Research
- Oncology
- Dermatology
Cite this
Acidic catalase in human skin in vivo : A new marker of permanent damage. / Maresca, Vittoria; Flori, Enrica; Fabbri, Claudia; Briganti, Stefania; Mariani, Giustino; Catricalà, Caterina; Picardo, Mauro.
In: Melanoma Research, Vol. 19, No. 6, 12.2009, p. 372-378.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Acidic catalase in human skin in vivo
T2 - A new marker of permanent damage
AU - Maresca, Vittoria
AU - Flori, Enrica
AU - Fabbri, Claudia
AU - Briganti, Stefania
AU - Mariani, Giustino
AU - Catricalà, Caterina
AU - Picardo, Mauro
PY - 2009/12
Y1 - 2009/12
N2 - Malignant melanoma incidence is increasing rapidly in Western countries. Its prevention requires a deep knowledge of the biological basis of the neoplasm leading to the identification of new biological risk markers. In in-vitro and ex-vivo models we demonstrated that catalase was modified not only in its activity but also in its charge properties after ultraviolet A irradiation through pheomelanin. Here we focus on the electrophoretic behaviour of catalase in the human skin in vivo, in association with cutaneous phototype. Zymographic analysis of the enzyme on skin biopsies from Caucasian population (phototype I-IV), collected from the trunk in autumn-winter, to exclude possible influences of an acute photoexposure, evidenced a protein doublet, representing the coexistence of two active isoforms of catalase with different charge properties. In the skin from low-phototype subjects, the percent contribution of the more acidic component of the doublet was prevalent, inversely correlated with total melanin concentration in hair, and associated with a high number of melanocytic nevi. In summary, this study shows for the first time the existence of an acidic catalase in association with clinically defined risk characteristics in low phototype skin in vivo, contributing to the knowledge of a new biochemical marker of cutaneous photosusceptibility. Melanoma Res 19:372-378
AB - Malignant melanoma incidence is increasing rapidly in Western countries. Its prevention requires a deep knowledge of the biological basis of the neoplasm leading to the identification of new biological risk markers. In in-vitro and ex-vivo models we demonstrated that catalase was modified not only in its activity but also in its charge properties after ultraviolet A irradiation through pheomelanin. Here we focus on the electrophoretic behaviour of catalase in the human skin in vivo, in association with cutaneous phototype. Zymographic analysis of the enzyme on skin biopsies from Caucasian population (phototype I-IV), collected from the trunk in autumn-winter, to exclude possible influences of an acute photoexposure, evidenced a protein doublet, representing the coexistence of two active isoforms of catalase with different charge properties. In the skin from low-phototype subjects, the percent contribution of the more acidic component of the doublet was prevalent, inversely correlated with total melanin concentration in hair, and associated with a high number of melanocytic nevi. In summary, this study shows for the first time the existence of an acidic catalase in association with clinically defined risk characteristics in low phototype skin in vivo, contributing to the knowledge of a new biochemical marker of cutaneous photosusceptibility. Melanoma Res 19:372-378
KW - Cutaneous phototype
KW - Hair melanin
KW - Native catalase
KW - Skin biopsies
UR - http://www.scopus.com/inward/record.url?scp=73349103765&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=73349103765&partnerID=8YFLogxK
U2 - 10.1097/CMR.0b013e3283312466
DO - 10.1097/CMR.0b013e3283312466
M3 - Article
VL - 19
SP - 372
EP - 378
JO - Melanoma Research
JF - Melanoma Research
SN - 0960-8931
IS - 6
ER -