Acidic catalase in human skin in vivo

A new marker of permanent damage

Vittoria Maresca, Enrica Flori, Claudia Fabbri, Stefania Briganti, Giustino Mariani, Caterina Catricalà, Mauro Picardo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Malignant melanoma incidence is increasing rapidly in Western countries. Its prevention requires a deep knowledge of the biological basis of the neoplasm leading to the identification of new biological risk markers. In in-vitro and ex-vivo models we demonstrated that catalase was modified not only in its activity but also in its charge properties after ultraviolet A irradiation through pheomelanin. Here we focus on the electrophoretic behaviour of catalase in the human skin in vivo, in association with cutaneous phototype. Zymographic analysis of the enzyme on skin biopsies from Caucasian population (phototype I-IV), collected from the trunk in autumn-winter, to exclude possible influences of an acute photoexposure, evidenced a protein doublet, representing the coexistence of two active isoforms of catalase with different charge properties. In the skin from low-phototype subjects, the percent contribution of the more acidic component of the doublet was prevalent, inversely correlated with total melanin concentration in hair, and associated with a high number of melanocytic nevi. In summary, this study shows for the first time the existence of an acidic catalase in association with clinically defined risk characteristics in low phototype skin in vivo, contributing to the knowledge of a new biochemical marker of cutaneous photosusceptibility. Melanoma Res 19:372-378

Original languageEnglish
Pages (from-to)372-378
Number of pages7
JournalMelanoma Research
Volume19
Issue number6
DOIs
Publication statusPublished - Dec 2009

Fingerprint

Catalase
Skin
Melanoma
Biomarkers
Pigmented Nevus
Melanins
Hair
Protein Isoforms
Biopsy
Incidence
Enzymes
Population
Neoplasms
Proteins

Keywords

  • Cutaneous phototype
  • Hair melanin
  • Native catalase
  • Skin biopsies

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Dermatology

Cite this

Acidic catalase in human skin in vivo : A new marker of permanent damage. / Maresca, Vittoria; Flori, Enrica; Fabbri, Claudia; Briganti, Stefania; Mariani, Giustino; Catricalà, Caterina; Picardo, Mauro.

In: Melanoma Research, Vol. 19, No. 6, 12.2009, p. 372-378.

Research output: Contribution to journalArticle

Maresca, Vittoria ; Flori, Enrica ; Fabbri, Claudia ; Briganti, Stefania ; Mariani, Giustino ; Catricalà, Caterina ; Picardo, Mauro. / Acidic catalase in human skin in vivo : A new marker of permanent damage. In: Melanoma Research. 2009 ; Vol. 19, No. 6. pp. 372-378.
@article{ece87189973f48919ea15ea907fe2179,
title = "Acidic catalase in human skin in vivo: A new marker of permanent damage",
abstract = "Malignant melanoma incidence is increasing rapidly in Western countries. Its prevention requires a deep knowledge of the biological basis of the neoplasm leading to the identification of new biological risk markers. In in-vitro and ex-vivo models we demonstrated that catalase was modified not only in its activity but also in its charge properties after ultraviolet A irradiation through pheomelanin. Here we focus on the electrophoretic behaviour of catalase in the human skin in vivo, in association with cutaneous phototype. Zymographic analysis of the enzyme on skin biopsies from Caucasian population (phototype I-IV), collected from the trunk in autumn-winter, to exclude possible influences of an acute photoexposure, evidenced a protein doublet, representing the coexistence of two active isoforms of catalase with different charge properties. In the skin from low-phototype subjects, the percent contribution of the more acidic component of the doublet was prevalent, inversely correlated with total melanin concentration in hair, and associated with a high number of melanocytic nevi. In summary, this study shows for the first time the existence of an acidic catalase in association with clinically defined risk characteristics in low phototype skin in vivo, contributing to the knowledge of a new biochemical marker of cutaneous photosusceptibility. Melanoma Res 19:372-378",
keywords = "Cutaneous phototype, Hair melanin, Native catalase, Skin biopsies",
author = "Vittoria Maresca and Enrica Flori and Claudia Fabbri and Stefania Briganti and Giustino Mariani and Caterina Catrical{\`a} and Mauro Picardo",
year = "2009",
month = "12",
doi = "10.1097/CMR.0b013e3283312466",
language = "English",
volume = "19",
pages = "372--378",
journal = "Melanoma Research",
issn = "0960-8931",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Acidic catalase in human skin in vivo

T2 - A new marker of permanent damage

AU - Maresca, Vittoria

AU - Flori, Enrica

AU - Fabbri, Claudia

AU - Briganti, Stefania

AU - Mariani, Giustino

AU - Catricalà, Caterina

AU - Picardo, Mauro

PY - 2009/12

Y1 - 2009/12

N2 - Malignant melanoma incidence is increasing rapidly in Western countries. Its prevention requires a deep knowledge of the biological basis of the neoplasm leading to the identification of new biological risk markers. In in-vitro and ex-vivo models we demonstrated that catalase was modified not only in its activity but also in its charge properties after ultraviolet A irradiation through pheomelanin. Here we focus on the electrophoretic behaviour of catalase in the human skin in vivo, in association with cutaneous phototype. Zymographic analysis of the enzyme on skin biopsies from Caucasian population (phototype I-IV), collected from the trunk in autumn-winter, to exclude possible influences of an acute photoexposure, evidenced a protein doublet, representing the coexistence of two active isoforms of catalase with different charge properties. In the skin from low-phototype subjects, the percent contribution of the more acidic component of the doublet was prevalent, inversely correlated with total melanin concentration in hair, and associated with a high number of melanocytic nevi. In summary, this study shows for the first time the existence of an acidic catalase in association with clinically defined risk characteristics in low phototype skin in vivo, contributing to the knowledge of a new biochemical marker of cutaneous photosusceptibility. Melanoma Res 19:372-378

AB - Malignant melanoma incidence is increasing rapidly in Western countries. Its prevention requires a deep knowledge of the biological basis of the neoplasm leading to the identification of new biological risk markers. In in-vitro and ex-vivo models we demonstrated that catalase was modified not only in its activity but also in its charge properties after ultraviolet A irradiation through pheomelanin. Here we focus on the electrophoretic behaviour of catalase in the human skin in vivo, in association with cutaneous phototype. Zymographic analysis of the enzyme on skin biopsies from Caucasian population (phototype I-IV), collected from the trunk in autumn-winter, to exclude possible influences of an acute photoexposure, evidenced a protein doublet, representing the coexistence of two active isoforms of catalase with different charge properties. In the skin from low-phototype subjects, the percent contribution of the more acidic component of the doublet was prevalent, inversely correlated with total melanin concentration in hair, and associated with a high number of melanocytic nevi. In summary, this study shows for the first time the existence of an acidic catalase in association with clinically defined risk characteristics in low phototype skin in vivo, contributing to the knowledge of a new biochemical marker of cutaneous photosusceptibility. Melanoma Res 19:372-378

KW - Cutaneous phototype

KW - Hair melanin

KW - Native catalase

KW - Skin biopsies

UR - http://www.scopus.com/inward/record.url?scp=73349103765&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73349103765&partnerID=8YFLogxK

U2 - 10.1097/CMR.0b013e3283312466

DO - 10.1097/CMR.0b013e3283312466

M3 - Article

VL - 19

SP - 372

EP - 378

JO - Melanoma Research

JF - Melanoma Research

SN - 0960-8931

IS - 6

ER -