TY - JOUR
T1 - Acidification prevents endothelial cell apoptosis by Axl activation.
AU - D'Arcangelo, Daniela
AU - Gaetano, Carlo
AU - Capogrossi, Maurizio C.
PY - 2002/10/4
Y1 - 2002/10/4
N2 - Prior studies have shown that acidification due to hypercarbia protects endothelial cells from serum deprivation-induced apoptosis. However, the mechanism(s) responsible for the antiapoptotic effect of acidification is still unclear. cDNA array screening was performed on human umbilical vein endothelial cells cultured in a bicarbonate medium equilibrated either with 5% CO2 (pH 7.4) or with 20% CO2 (pH 7.0). Tyrosine kinase receptor Axl expression was 3.3-fold higher after 6 hours at pH 7.0 compared with pH 7.4; this modulation was confirmed by reverse transcriptase-polymerase chain reaction (3.0+/-0.9-fold, P
AB - Prior studies have shown that acidification due to hypercarbia protects endothelial cells from serum deprivation-induced apoptosis. However, the mechanism(s) responsible for the antiapoptotic effect of acidification is still unclear. cDNA array screening was performed on human umbilical vein endothelial cells cultured in a bicarbonate medium equilibrated either with 5% CO2 (pH 7.4) or with 20% CO2 (pH 7.0). Tyrosine kinase receptor Axl expression was 3.3-fold higher after 6 hours at pH 7.0 compared with pH 7.4; this modulation was confirmed by reverse transcriptase-polymerase chain reaction (3.0+/-0.9-fold, P
UR - http://www.scopus.com/inward/record.url?scp=0037019946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037019946&partnerID=8YFLogxK
M3 - Article
C2 - 12364394
AN - SCOPUS:0037019946
VL - 91
JO - Circulation Research
JF - Circulation Research
SN - 0009-7330
IS - 7
ER -