Abstract
Myocardial acidosis, as during ischemia, profoundly modifies excitation-contraction mechanisms. The decreased myofilament sensitivity to Ca2+ reduces contractility regardless of an intracellular accumulation of Ca2+. To determine the source for this increase in Ca2+ we evaluated the effect of acidosis on diastolic [Ca2+] and mitochondrial [Ca2+]. We used single cardiac cells loaded with the fluorescent probes, indo-1 for Ca2+ and SNARF-1 for pH. Acidosis increases [Ca2+] both in cytosol and mitochondria. The cytosolic accumulation depends, most likely, on an active release from mitochondria. A competition among Ca2+ and H+ ions may, instead, explains the increase in mitochondrial [Ca2+].
Translated title of the contribution | Acidosis is associated with an intracellular accumulation of Ca2+. Its role in the modulation of myocardial contractility |
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Original language | Italian |
Pages (from-to) | 587-589 |
Number of pages | 3 |
Journal | Cardiologia |
Volume | 37 |
Issue number | 8 |
Publication status | Published - Aug 1992 |
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine