Acquisition of high metastatic capacity after in vitro fusion of a nonmetastatic tumor line with a bone marrow-derived macrophage

L. Larizza, V. Schirrmacher, E. Pfluger

Research output: Contribution to journalArticlepeer-review

Abstract

A low metastatic, thioguanine-resistant murine T lymphoma line (Eb(TGR)) was hybridized in vitro, with the help of polyethylene glycol, with syngeneic bone marrow-derived macrophages. Two HAT-resistant hybrid lines (Eb-F1 and Eb-F2) were obtained from independent fusion cultures. A cytogenetic analysis revealed that most of the macrophage chromosome except No. 12 had segregated or become rearranged 60 d after fusion, a time at which the cell lines had become stabilized in culture. Syngeneic mice inoculated subcutaneously with the tumor macrophage hybrid lines developed, very quickly, visceral metastases and died after 6 wk and developed only localized, large primary tumors. The metastatic hybridomas expressed a similar tumor antigen as a spontaneous, in vivo derived, high metastatic variant (ESb) of the same tumor. This suggests that ESb cells might have arisen from a spontaneous fusion with a host macrophage.

Original languageEnglish
Pages (from-to)1579-1584
Number of pages6
JournalJournal of Experimental Medicine
Volume160
Issue number5
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Immunology

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