Acquisition of intact allogeneic human leukocyte antigen molecules by human dendritic cells

Vincenzo Russo, Dan Zhou, Claudia Sartirana, Patrizia Rovere, Antonello Villa, Silvano Rossini, Catia Traversari, Claudio Bordignon

Research output: Contribution to journalArticlepeer-review

Abstract

In an attempt to transduce monocyte-derived dendritic cells (DCs) by a retroviral vector coding for a cell surface marker, we were confronted by the observation of high transfer of the surface molecule in the absence of vector proviral DNA in the treated cells. Indeed, DCs acquired the surface marker by a mechanism independent of the vector machinery, requiring cell-to-cell contact and involving transfer of lipids and a variety of intact membrane proteins. Most important, this property of DCs also includes acquisition of foreign human leukocyte antigen (HLA) molecules. Consequently, DCs become immunological hybrids as they display their own and foreign HLA molecules. The newly acquired HLA is fully functional because it allows recognition by allo-specific T lymphocytes and the binding and presentation of antigen peptides. (C) 2000 by The American Society of Hematology.

Original languageEnglish
Pages (from-to)3473-3477
Number of pages5
JournalBlood
Volume95
Issue number11
Publication statusPublished - Jun 1 2000

ASJC Scopus subject areas

  • Hematology

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