Acridine orange/exosomes increase the delivery and the effectiveness of acridine orange in human melanoma cells: A new prototype for theranostics of tumors

Elisabetta Iessi, Mariantonia Logozzi, Luana Lugini, Tommaso Azzarito, Cristina Federici, Enrico Pierluigi Spugnini, Davide Mizzoni, Rossella Di Raimo, Daniela F. Angelini, Luca Battistini, Serena Cecchetti, Stefano Fais

Research output: Contribution to journalArticle

Abstract

Specifically targeted drug delivery systems with low immunogenicity and toxicity are deemed to increase efficacy of cancer chemotherapy. Acridine Orange (AO) is an acidophilic dye with a strong tumoricidal action following excitation with a light source at 466 nm. However, to date the clinical use of AO is limited by the potential side effects elicited by systemic administration. The endogenous nanocarrier exosomes have been recently introduced as a natural delivery system for therapeutic molecules. In this article, we show the outcome of the administration to human melanoma cells of AO charged Exosomes (Exo-AO), in both monolayer and spheroid models. The results showed an extended drug delivery time of Exo-AO to melanoma cells as compared to the free AO, improving the cytotoxicity of AO. This study shows that Exo- AO have a great potential for a real exploitation as a new theranostic approach against tumors based on AO delivered through the exosomes.

Original languageEnglish
Pages (from-to)648-657
Number of pages10
JournalJournal of Enzyme Inhibition and Medicinal Chemistry
Volume32
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Keywords

  • Acridine orange
  • Delivery system
  • Exosome
  • Macrophage
  • Theranostics

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Fingerprint Dive into the research topics of 'Acridine orange/exosomes increase the delivery and the effectiveness of acridine orange in human melanoma cells: A new prototype for theranostics of tumors'. Together they form a unique fingerprint.

  • Cite this