Acridine orange/exosomes increase the delivery and the effectiveness of acridine orange in human melanoma cells: A new prototype for theranostics of tumors

E. Iessi, M. Logozzi, L. Lugini, T. Azzarito, C. Federici, E.P. Spugnini, D. Mizzoni, R. Di Raimo, D.F. Angelini, L. Battistini, S. Cecchetti, S. Fais

Research output: Contribution to journalArticle

Abstract

Specifically targeted drug delivery systems with low immunogenicity and toxicity are deemed to increase efficacy of cancer chemotherapy. Acridine Orange (AO) is an acidophilic dye with a strong tumoricidal action following excitation with a light source at 466 nm. However, to date the clinical use of AO is limited by the potential side effects elicited by systemic administration. The endogenous nanocarrier exosomes have been recently introduced as a natural delivery system for therapeutic molecules. In this article, we show the outcome of the administration to human melanoma cells of AO charged Exosomes (Exo-AO), in both monolayer and spheroid models. The results showed an extended drug delivery time of Exo-AO to melanoma cells as compared to the free AO, improving the cytotoxicity of AO. This study shows that Exo- AO have a great potential for a real exploitation as a new theranostic approach against tumors based on AO delivered through the exosomes. © 2017 The Author(s).
Original languageEnglish
Pages (from-to)648-657
Number of pages10
JournalJournal of Enzyme Inhibition and Medicinal Chemistry
Volume32
Issue number1
DOIs
Publication statusPublished - 2017

Keywords

  • Acridine orange
  • Delivery system
  • Exosome
  • Macrophage
  • Theranostics
  • acridine orange
  • antineoplastic agent
  • Article
  • controlled study
  • cytotoxicity
  • drug delivery system
  • drug formulation
  • exosome
  • melanoma cell
  • metastatic melanoma
  • priority journal
  • theranostic nanomedicine
  • chemistry
  • confocal microscopy
  • flow cytometry
  • human
  • melanoma
  • pH
  • tumor cell line
  • Acridine Orange
  • Antineoplastic Agents
  • Cell Line, Tumor
  • Drug Delivery Systems
  • Exosomes
  • Flow Cytometry
  • Humans
  • Hydrogen-Ion Concentration
  • Melanoma
  • Microscopy, Confocal
  • Theranostic Nanomedicine

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