Actin cytoskeleton as a target for 2-chloro adenosine: Evidence for induction of apoptosis in C2C12 myoblastic cells

Stefano Rufini, Gabriella Rainaldi, Maria Pia Abbracchio, Carla Fiorentini, Miriam Capri, Claudio Franceschi, Walter Malorni

Research output: Contribution to journalArticle

Abstract

Recent results for various cell types have shown that adenosine is capable of inducing relevant cytophatological alterations which can eventually lead to apoptosis. No data are available regarding the involvement of adenosine in apoptosis of muscle cells. In this work, we studied the effect of the relatively hydrolysis-resistant adenosine analog 2-chloro adenosine on a cultured myogenic cell line, C2C12, which is able to differentiate in vitro, leading to the formation of syncythia, giant multinucleated cells called myotubes. Results indicated that 2-chloro adenosine induces apoptotic cell death in both myoblasts and myotubes; this was preceded by a derangement of actin microfilaments, which represent the main cytoskeletal component and play a pivotal role in the physiology of such cell line. The time dependency of cytoskeletal alterations suggested a causal relationship between these changes and the induction of apoptosis. These results implicate adenosine as an endogenous regulator of apoptosis in muscle cells and validate this cell model system as a useful tool for studying human muscle cell pathologies.

Original languageEnglish
Pages (from-to)361-366
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume238
Issue number2
DOIs
Publication statusPublished - Sep 18 1997

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Actin cytoskeleton as a target for 2-chloro adenosine: Evidence for induction of apoptosis in C2C12 myoblastic cells'. Together they form a unique fingerprint.

  • Cite this