Azione del retinolo sul recupero di cellule vitali e sul potenziale clonogenico di cellule HTC ipertermizzate in vitro.

Translated title of the contribution: Action of retinol on viable cell recovery and clonogenic potential of HTC cells after in vitro hyperthermia

D. Càllari, G. Blandino, V. Saccone, C. D'Amico, A. Billitteri

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to investigate if the association of both hyperthermic and Retinol treatment of HTC hepatoma cells could be useful in antitumor therapy. Treatment with 5 microM Retinol was carried out before or after hyperthermia (42 degrees C or 44 degrees C, one hour; in the latter case it was performed in cells already thermo-selected. We took into consideration two parameters, i.e. the number of the collected vital cells (evaluated by the trypan blue-exclusion test) and the clonal efficiency of these cells (calculated as number of colonies obtained from 250 cells cultured for 5 days). Thermal treatment alone caused a decrease of the number of the collected vital cells and of their clonal efficiency only in the cell cultures incubated at 44 degrees C. Instead the control thermo-selected cells, both at 42 degrees C and at 44 degrees C, showed both decreased clonal efficiency and yield of the vital cells. Compared with the control cultures treated with 0.1% Ethanol, used as vitamin A solvent, only cell cultures treated with Retinol before hyperthermia showed a decreased number of collected viable cells, nevertheless their clonal efficiency was unchanged.

Translated title of the contributionAction of retinol on viable cell recovery and clonogenic potential of HTC cells after in vitro hyperthermia
Original languageItalian
Pages (from-to)783-787
Number of pages5
JournalBollettino della Societa Italiana di Biologia Sperimentale
Volume68
Issue number12
Publication statusPublished - Dec 1992

ASJC Scopus subject areas

  • Medicine(all)

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