Abstract
The aim of this study was to investigate if the association of both hyperthermic and Retinol treatment of HTC hepatoma cells could be useful in antitumor therapy. Treatment with 5 microM Retinol was carried out before or after hyperthermia (42 degrees C or 44 degrees C, one hour; in the latter case it was performed in cells already thermo-selected. We took into consideration two parameters, i.e. the number of the collected vital cells (evaluated by the trypan blue-exclusion test) and the clonal efficiency of these cells (calculated as number of colonies obtained from 250 cells cultured for 5 days). Thermal treatment alone caused a decrease of the number of the collected vital cells and of their clonal efficiency only in the cell cultures incubated at 44 degrees C. Instead the control thermo-selected cells, both at 42 degrees C and at 44 degrees C, showed both decreased clonal efficiency and yield of the vital cells. Compared with the control cultures treated with 0.1% Ethanol, used as vitamin A solvent, only cell cultures treated with Retinol before hyperthermia showed a decreased number of collected viable cells, nevertheless their clonal efficiency was unchanged.
| Translated title of the contribution | Action of retinol on viable cell recovery and clonogenic potential of HTC cells after in vitro hyperthermia |
|---|---|
| Original language | Italian |
| Pages (from-to) | 783-787 |
| Number of pages | 5 |
| Journal | Bollettino della Societa Italiana di Biologia Sperimentale |
| Volume | 68 |
| Issue number | 12 |
| Publication status | Published - Dec 1992 |
ASJC Scopus subject areas
- Medicine(all)