Action potential detection by non linear microscopy

Leonardo Sacconi, Jacopo Lotti, Rodney P. O'Connor, Jonathan Mapelli, Daniela Gandolfi, Egidio D'Angelo, Francesco S. Pavone

Research output: Chapter in Book/Report/Conference proceedingConference contribution


In this work, we combined the advantages of second-harmonic generation (SHG) with a random access (RA) excitation scheme to realize a new microscope (RA-SHG) capable of optically recording fast membrane potential events occurring in a wide-field configuration. The RA-SHG microscope in combination with a bulk staining method with FM4-64 was used to simultaneously record electrical activity from clusters of Purkinje cells (PCs) in acute cerebellar slices. Spontaneous electrical activity was also monitored simultaneously in pairs of neurons, where APs were recorded in a single trial without averaging. These results show the strength of this technique to describe the temporal dynamics of neuronal assemblies.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Publication statusPublished - 2009
EventPhotonic Therapeutics and Diagnostics V - San Jose, CA, United States
Duration: Jan 24 2009Jan 26 2009


OtherPhotonic Therapeutics and Diagnostics V
Country/TerritoryUnited States
CitySan Jose, CA


  • Action potential
  • Cerebellum
  • Non-linear microscopy
  • Purkinje cell

ASJC Scopus subject areas

  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Applied Mathematics
  • Condensed Matter Physics


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