Action potentials recorded with patch-clamp amplifiers: Are they genuine?

Jacopo Magistretti, Massimo Mantegazza, Ezia Guatteo, Enzo Wanke

Research output: Contribution to journalArticlepeer-review

Abstract

A growing number of experimental studies have used patch-clamp amplifiers (PCAs) in the current-clamp (CC) mode to investigate classical excitability. In this paper we show that the measurements obtained in this way are affected by errors due to the electronic design of the PCA input section. We present experimental evidence of such errors, and demonstrate that they derive from PCA current absorption. Moreover, we propose a new PCA input-circuit configuration for the CC mode, which is suitable for accurately recording physiological voltage signals and is perfectly compatible with the standard voltage-clamp mode.

Original languageEnglish
Pages (from-to)530-534
Number of pages5
JournalTrends in Neurosciences
Volume19
Issue number12
DOIs
Publication statusPublished - Dec 1996

ASJC Scopus subject areas

  • Neuroscience(all)

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