Azione del retinolo e dell'acido retinoico su colture di epatoma H4.

Translated title of the contribution: Actions of retinol and retinoic acid on hepatoma H4 cultures

D. Càllari, G. Gasso, G. Blandino, A. Billitteri

Research output: Contribution to journalArticle


The amount of vital cells recovered, their morphology (studied by SEM) and some of their biochemical aspects concerning the differentiation processes (aerobic glycolysis, cell production of cAMP and CEA) were investigated in a strain of H4 hepatoma cultured for 8 days in the presence of 5 microM retinol (R) or retinoic Acid (RA). Vital cell recovery is slightly reduced either by R or RA treatment. Flattening of the cell shape and reduction of the plasma membrane prolongations and of intercellular bonds are observed in the R-treated cells but to a greater extent in those treated with RA. Aerobic glycolysis is decreased in the R-treated cells but increased in those treated with RA. Such events could be related to the regressive processes observed in the RA-treated cells. cAMP cell content is increased to a greater extent in the R-treated cells than in those treated with RA. CEA cell content is greatly decreased in the RA-treated cells but only slightly in those treated with R. Therefore, the treatment of HA hepatoma cells with 5 microM R or RA, while reducing cell growth only slightly, does not cause evident and unequivocal morphological and biochemical events related to cell redifferentiation.

Original languageItalian
Pages (from-to)1081-1087
Number of pages7
JournalBollettino della Societa Italiana di Biologia Sperimentale
Issue number11
Publication statusPublished - Nov 1989


ASJC Scopus subject areas

  • Medicine(all)

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