Activated B lymphocytes from human immunodeficiency virus-infected individuals induce virus expression in infected T cells and a promonocytic cell line, U1

Peter Rieckmann, Guido Poli, John H. Kehrl, Anthony S. Fauci

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Abstract

Freshly isolated B lymphocytes from patients infected with human immunodeficiency virus (HIV), in contrast to B cells from normal controls, were shown to induce viral expression in two cell lines: ACH-2, a T cell line, and U1, a promonocytic cell line, which are chronically infected with HIV, as well as in autologous T cells. In 10 out of 10 HIV-infected individuals with hypergammaglobulinemia, spontaneous HIV-inductive capacity was found with highly purified peripheral blood B cells, whereas peripheral blood or tonsillar B cells from six healthy, HIV-negative donors did not induce HIV expression unless the cells were stimulated in vitro. The induction of HIV expression was observed in direct coculture experiments of B lymphocytes and HIV-infected cells, and could also be mediated by supernatants from cultures of B cells. Significantly higher amounts of interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) were detected in the B cell culture supernatants from HIV-infected patients with hypergammaglobulinemia (IL-6: x̄ = 536 pg/ml; TNF-α: x̄ = 493 pg/ml), as compared with normal uninfected controls (IL-6: x̄ = 18 pg/ml; TNF-α: x̄ = 23 pg/ml). Antibodies against these cytokines abolished the HIV-inductive capacity of B cells. We conclude that in vivo activated B cells in HIV-infected individuals can upregulate the expression of virus in infected cells by secreting cytokines such as TNF-α and IL-6, and, therefore, may play a role in the progression of HIV infection.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalJournal of Experimental Medicine
Volume173
Issue number1
Publication statusPublished - 1991

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B-Lymphocytes
HIV
Viruses
T-Lymphocytes
Cell Line
Interleukin-6
Hypergammaglobulinemia
Cytokines
Virus Diseases
Coculture Techniques
Blood Cells
Up-Regulation
Cell Culture Techniques
Tumor Necrosis Factor-alpha
Tissue Donors
Antibodies

ASJC Scopus subject areas

  • Immunology

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Activated B lymphocytes from human immunodeficiency virus-infected individuals induce virus expression in infected T cells and a promonocytic cell line, U1. / Rieckmann, Peter; Poli, Guido; Kehrl, John H.; Fauci, Anthony S.

In: Journal of Experimental Medicine, Vol. 173, No. 1, 1991, p. 1-5.

Research output: Contribution to journalArticle

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