Activated lymphoid cells in human gliomas: Morphofuctional and cytochemical evidence

Rosanna Nano, Enrica Capelli, Monica Civallero, Lorenzo Lorusso, Fabio Argentina, Ennio Bonizzoni, Mauro Ceroni

Research output: Contribution to journalArticlepeer-review


To study defensive infiltrating cells, smear protection from 20 fresh gliomas and autologous normal peritumoural brain tissue, used as control, were analysed. May-Grunwald Giemsa staining and cytochemical reaction markers of cellular functions [acid phosphatase (AP), dihydrofolate reductase (DHFR), dipeptidilpeptidase (DAPIV) and serine esterase BLT-dependent (SE)], were employed to characterize the cells. The extent of the leukocytic infiltration and the percentage of activated lymphocytes ('hand mirror' shape lymphocytes: HMS; lymphocytes binding tumour: LBT) were also studied. In addition serum levels of T cell growth factor interleukin-2 (IL-2) and of the soluble IL-2 receptor (sIL-2R) were determined in the affected patients. In tumoural imprints, mostly in astrocytomas, we observed an increased percentage of DHFR and DAP IV positive lymphocytes, of HMS lymphocytes and of lymphocytes binding tumoral cells. Serum levels of IL-2 were significantly increased in all patients whilst sIL-2R levels were high only in glioblastoma. These findings indicate that in malignant gliomas there is stimulation of immune system as witnessed by the presence of activated cells inside tumour tissue and soluble activating factors in serum.

Original languageEnglish
Pages (from-to)107-111
Number of pages5
JournalAnticancer Research
Issue number1 A
Publication statusPublished - 1997


  • Activated cells
  • Cytochemistry
  • Cytokines
  • Glioma
  • Immunoassay

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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