Activation by point mutation of Ki-ras gene occurring in transfected human normal DNA

A. Viel, R. Maestro, M. Pistello, R. Dolcetti, V. De Re, M. Boiocchi

Research output: Contribution to journalArticlepeer-review

Abstract

A mutation-activated human Ki-ras gene was detected in NIH 3T3 cells transfected with high molecular weight DNA extracted from peripheral blood leukocytes of a healthy blood donor. A deoxyguanosine at position 35 of the first exon was substituted by deoxythymidine. Nevertheless, cloning and sequencing of seven independent Ki-ras first exons, isolated from the same human genomic DNA used to transfect NIH 3T3 cells, failed to reveal the expected point mutation. Since transfected DNA is susceptible to mutagenesis in mammalian cells, we hypothesize that a base substitution occurred during the transfection assay.

Original languageEnglish
Pages (from-to)499-506
Number of pages8
JournalTumori
Volume74
Issue number5
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Cancer Research

Fingerprint Dive into the research topics of 'Activation by point mutation of Ki-ras gene occurring in transfected human normal DNA'. Together they form a unique fingerprint.

Cite this