Activation of arachidonic acid metabolism during aggregation of polymorphonuclear granulocytes

G. Ricevuti; L. Pacchiarini; A. Mazzone; D. Rota Scalabrini; G. Grignani; S. C. Rizzo

Activation of arachidonic acid metabolism during aggregation of polymorphonuclear granulocytes

G. Ricevuti, L. Pacchiarini, A. Mazzone, D. Rota Scalabrini, G. Grignani, S. C. Rizzo

Istituto Oncologico Candiolo

Research output: Contribution to journal › Article

Abstract

The role of neutrophil aggregation in arachidonic acid metabolism and of the phospholipid components of the cellular membrane were investigated. PMNs aggregated in response to different substances (PHA, PMA, FMLP, Thrombin, Trypsin). PHA-induced aggregation was associated with the production of TxB₂ and LTC₄; ASA at 0.3, 0.5 and 1.0 mM concentrations significantly decreased neutrophil aggregation and TxB₂ production and slightly increased LTC₄ production. Phospholipases A₂, C and D preincubated with PMN suspension reduced PHA-induced aggregation. These results suggest that the activation of arachidonic acid metabolism is involved in PMN aggregation and ASA seems to modify only the prostaglandin biosynthesis pathway. The phospholipid components of the cellular membrane are essential in the aggregation of stimulated PMNs.

Original language	English
Pages (from-to)	329-339
Number of pages	11
Journal	Hematology Reviews and Communications
Volume	1
Issue number	4
Publication status	Published - 1987

ASJC Scopus subject areas

Hematology

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Ricevuti, G., Pacchiarini, L., Mazzone, A., Rota Scalabrini, D., Grignani, G., & Rizzo, S. C. (1987). Activation of arachidonic acid metabolism during aggregation of polymorphonuclear granulocytes. Hematology Reviews and Communications, 1(4), 329-339.

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abstract = "The role of neutrophil aggregation in arachidonic acid metabolism and of the phospholipid components of the cellular membrane were investigated. PMNs aggregated in response to different substances (PHA, PMA, FMLP, Thrombin, Trypsin). PHA-induced aggregation was associated with the production of TxB2 and LTC4; ASA at 0.3, 0.5 and 1.0 mM concentrations significantly decreased neutrophil aggregation and TxB2 production and slightly increased LTC4 production. Phospholipases A2, C and D preincubated with PMN suspension reduced PHA-induced aggregation. These results suggest that the activation of arachidonic acid metabolism is involved in PMN aggregation and ASA seems to modify only the prostaglandin biosynthesis pathway. The phospholipid components of the cellular membrane are essential in the aggregation of stimulated PMNs.",

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T1 - Activation of arachidonic acid metabolism during aggregation of polymorphonuclear granulocytes

AU - Ricevuti, G.

AU - Pacchiarini, L.

AU - Mazzone, A.

AU - Rota Scalabrini, D.

AU - Grignani, G.

AU - Rizzo, S. C.

PY - 1987

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AB - The role of neutrophil aggregation in arachidonic acid metabolism and of the phospholipid components of the cellular membrane were investigated. PMNs aggregated in response to different substances (PHA, PMA, FMLP, Thrombin, Trypsin). PHA-induced aggregation was associated with the production of TxB2 and LTC4; ASA at 0.3, 0.5 and 1.0 mM concentrations significantly decreased neutrophil aggregation and TxB2 production and slightly increased LTC4 production. Phospholipases A2, C and D preincubated with PMN suspension reduced PHA-induced aggregation. These results suggest that the activation of arachidonic acid metabolism is involved in PMN aggregation and ASA seems to modify only the prostaglandin biosynthesis pathway. The phospholipid components of the cellular membrane are essential in the aggregation of stimulated PMNs.

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