Activation of CD3/TCR negative human thymocytes via CD28 molecule

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Abstract

We have observed that the CD28 molecule was present on the cell surface of a large fraction of resting CD3- thymocytes (40 to 100%). Interestingly, the majority (>90%) of surface CD3-CD28- cells reacted in the cytoplasm with anti-CD28 (CK248, 9.3) and anti-CD3ε chain mAbs (Leu4, OKT3). Along this line, we found that CD28 surface expression could be induced within 18 hr on CD3-CD28- thymocytes using very low doses of phorbol-13-myristate-12-acetate (PMA). This event was accompanied by the appearance of CD25 and CD69 activation antigens but not of CD3/TCR complex. These results were further confirmed by immunoprecipitation studies. It is noteworthy that the T-cell activation pathway initiated via the CD28 molecule is functional in resting CD3- thymocytes in the presence of PMA and/or IL2. Finally, stimulation of CD3- immature thymocytes via CD28 gave rise to a large fraction (about one-third) of CD3-CD8+ cells.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalCellular Immunology
Volume136
Issue number1
DOIs
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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