The percentages of total (EG1+) and activated (EG2+) eosinophils and serum levels of eosinophil cationic protein (ECP) and eosinophilic protein-X (EPX) were determined in peripheral blood samples taken from patients with 3 types of allergic skin disorders, adult atopic dermatitis (AD), contact dermatitis (DAC) and urticaria/angioedema (SOA) and a group of healthy non-allergic adult controls to evaluate the contribution of activated circulating eosinophils to the pathogenesis of these conditions. Eosinophils were isolated by the FOG method, stained with the monoclonal antibodies EG1+ and EG2+ and then enumerated by flow cytometry. The percentages of EG1+ and EG2+ cells, peripheral blood eosinophil counts and serum levels of both ECP and EPX were significantly higher in the groups with DA and DAC than in the controls but not in those with SOA. When the mean values of the same parameters were calculated after subdividing each diagnostic group according to symptom severity, a tendency towards an increase in each of the parameters was observed with increasing severity within each diagnostic group but significant differences were reached for all parameters only in the DA group, only for the percent of EG2+ cells in those with DAC and for serum ECP levels alone in the group with SOA. These data suggest a possible pathogenetic role for circulating eosinophils in the allergic skin disorders.
|Translated title of the contribution||Activation of circulating eosinophils in patients with allergic skin disorders|
|Number of pages||5|
|Journal||Giornale Italiano di Dermatologia e Venereologia|
|Publication status||Published - 1996|
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