Abstract
(RS)-α-Methyl-4-phosphonophenylglycine (MPPG) and (S)-α-methyl-3-carboxyphenylalanine (M3CPA), two novel preferential antagonists of group III metabotropic glutamate (mGlu) receptors, antagonized the neuroprotective activity of L-2-amino-4-phosphonobutanoate (L-AP4) or L-serine-O-phosphate in mice cultured cortical cells exposed to a toxic pulse of N-methyl-D-aspartate. In contrast, MPPG did not influence the neuroprotective activity of the selective group II mGlu receptors agonist, (2(S),1'(R),2'(R),3'(R))-2-(2,3-dicarboxycyclopropyl) glycine (DCG-IV). These results indicate that activation of group m mGlu receptors exerts neuroprotective activity against excitotoxic neuronal death. At least one of the two major group III mGlu receptor subtypes, i.e. mGlu4 receptor, is expressed by cultured cortical neurons, as shown by immunocytochemical analysis with specific polyclonal antibodies.
| Original language | English |
|---|---|
| Pages (from-to) | 61-66 |
| Number of pages | 6 |
| Journal | European Journal of Pharmacology |
| Volume | 310 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Aug 22 1996 |
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Keywords
- Cortical neuron
- Excitotoxicity
- Metabotropic glutamate receptor
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Pharmacology
Cite this
Activation of group III metabotropic glutamate receptors is neuroprotective in cortical cultures. / Bruno, V.; Copani, A.; Bonanno, L.; Knoepfel, T.; Kuhn, R.; Roberts, P. J.; Nicoletti, F.
In: European Journal of Pharmacology, Vol. 310, No. 1, 22.08.1996, p. 61-66.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Activation of group III metabotropic glutamate receptors is neuroprotective in cortical cultures
AU - Bruno, V.
AU - Copani, A.
AU - Bonanno, L.
AU - Knoepfel, T.
AU - Kuhn, R.
AU - Roberts, P. J.
AU - Nicoletti, F.
PY - 1996/8/22
Y1 - 1996/8/22
N2 - (RS)-α-Methyl-4-phosphonophenylglycine (MPPG) and (S)-α-methyl-3-carboxyphenylalanine (M3CPA), two novel preferential antagonists of group III metabotropic glutamate (mGlu) receptors, antagonized the neuroprotective activity of L-2-amino-4-phosphonobutanoate (L-AP4) or L-serine-O-phosphate in mice cultured cortical cells exposed to a toxic pulse of N-methyl-D-aspartate. In contrast, MPPG did not influence the neuroprotective activity of the selective group II mGlu receptors agonist, (2(S),1'(R),2'(R),3'(R))-2-(2,3-dicarboxycyclopropyl) glycine (DCG-IV). These results indicate that activation of group m mGlu receptors exerts neuroprotective activity against excitotoxic neuronal death. At least one of the two major group III mGlu receptor subtypes, i.e. mGlu4 receptor, is expressed by cultured cortical neurons, as shown by immunocytochemical analysis with specific polyclonal antibodies.
AB - (RS)-α-Methyl-4-phosphonophenylglycine (MPPG) and (S)-α-methyl-3-carboxyphenylalanine (M3CPA), two novel preferential antagonists of group III metabotropic glutamate (mGlu) receptors, antagonized the neuroprotective activity of L-2-amino-4-phosphonobutanoate (L-AP4) or L-serine-O-phosphate in mice cultured cortical cells exposed to a toxic pulse of N-methyl-D-aspartate. In contrast, MPPG did not influence the neuroprotective activity of the selective group II mGlu receptors agonist, (2(S),1'(R),2'(R),3'(R))-2-(2,3-dicarboxycyclopropyl) glycine (DCG-IV). These results indicate that activation of group m mGlu receptors exerts neuroprotective activity against excitotoxic neuronal death. At least one of the two major group III mGlu receptor subtypes, i.e. mGlu4 receptor, is expressed by cultured cortical neurons, as shown by immunocytochemical analysis with specific polyclonal antibodies.
KW - Cortical neuron
KW - Excitotoxicity
KW - Metabotropic glutamate receptor
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UR - http://www.scopus.com/inward/citedby.url?scp=0030598124&partnerID=8YFLogxK
U2 - 10.1016/0014-2999(96)00358-5
DO - 10.1016/0014-2999(96)00358-5
M3 - Article
VL - 310
SP - 61
EP - 66
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1
ER -