Activation of human B-MYB by cyclins

Arturo Sala, Mondira Kundu, Ida Casella, Andrew Engelhard, Bruno Calabretta, Luigi Grasso, Marco G. Paggi, Antonio Giordano, Roger J. Watson, Kamel Khalili, Cesare Peschle

Research output: Contribution to journalArticle

Abstract

B-MYB expression is associated with cell proliferation and recent studies have suggested that it promotes the S phase of mammalian cells. Based on its homology to the transcription factors c-MYB and A-MYB, B-MYB is thought to be involved in transcriptional regulation; however, its activity is not detectable in several cell lines. It was postulated that B-MYB function may depend on the presence of a cofactor, and recent studies suggested that B-MYB is phosphorylated specifically during S phase in murine fibroblasts. In this report we provide evidence that the product of the human B-myb gene can be activated in vivo by coexpression with cyclin A or cyclin E. Transfection studies showed that B-MYB was a weak transcriptional activator in SAOS-2 cells and was unable to promote their proliferation. In contrast, overexpression of both B-MYB and cyclin A or cyclin E caused a drastic increase in the number of SAOS-2 cells in S phase. Also, overexpression of cyclin A and cyclin E in SAOS-2 cells enhanced the ability of B-MYB, but not c-MYB, to transactivate various promoters, including the cdc2 promoter, the HIV-1-LTR, and the simian virus 40 minimal promoter. A direct role for cyclin-dependent activation of B-MYB was demonstrated using an in vitro transcription assay. These observations suggest that one mechanism by which cyclin A and E may promote the S phase is through modification and activation of B-MYB.

Original languageEnglish
Pages (from-to)532-536
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number2
DOIs
Publication statusPublished - Jan 21 1997

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Keywords

  • cell cycle
  • cyclin A
  • cyclin E
  • phosphorylation
  • transactivation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Sala, A., Kundu, M., Casella, I., Engelhard, A., Calabretta, B., Grasso, L., Paggi, M. G., Giordano, A., Watson, R. J., Khalili, K., & Peschle, C. (1997). Activation of human B-MYB by cyclins. Proceedings of the National Academy of Sciences of the United States of America, 94(2), 532-536. https://doi.org/10.1073/pnas.94.2.532