Activation of human basophils by staphylococcal protein A. I. The role of cyclic AMP, arachidonic acid metabolites, microtubules and microfilaments

G. Marone, S. Poto, R. Petracca, M. Triggiani, E. de Lutio di Castelguidone, M. Condorelli

Research output: Contribution to journalArticlepeer-review

Abstract

Protein A from Staphylococcus aureus (Staph a) induces histamine secretion from human basophil leucocytes in the concentration range 10 -4-10 μg/ml. This reaction has great similarities to that of antigen or anti-IgE-induced release. It is characterized by a two stage reaction, requires extracellular calcium and is optimal at 37°C. The rate of release is similar to that of IgE-mediated reactions. Histamine release induced by Staph A is inhibited by metabolic inhibitors, drugs which increase intracellular cyclic AMP levels, inhibitors of lipoxygenase pathways and a phospholipase A 2 inhibitor, D 2O and cytochalasin B which affect microtubules and microfilaments respectively, enhance histamine release induced by Staph A. These results suggest that Staph A-induced release is modulated by intracellular cyclic AMP, arachidonic acid metabolites, requires energy and is enhanced by the disruption of microgifaments and stabilization of microtubules.

Original languageEnglish
Pages (from-to)661-668
Number of pages8
JournalClinical and Experimental Immunology
Volume50
Issue number3
Publication statusPublished - 1982

ASJC Scopus subject areas

  • Immunology

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