Activation of human platelets by lysophosphatidic acid

M. Torti, E. Tolnai Festetics, A. Bertoni, F. Sinigaglia, C. Balduini

Research output: Contribution to journalArticle

Abstract

The biochemical mechanisms of platelet activation by lysophosphatidic acid were investigated. Lysophosphatidic acid interacts with a membrane receptor coupled to the inhibitory GTP-binding protein Gi and produces a rapid decrease of the intracellular concentration of cAMP. Aggregation of gel-filtered platelets by lysophosphatidic acid requires the presence of extracellular CaCl2, as this phospholipid does not induce secretion of platelet dense granules. Platelet activation by lysophosphatidic acid in the absence of extracellular CaCl2 does not involve phospholipase C activation, Is evaluated by measuring mobilization of Ca2+ from internal stores and pleckstrin phosphorylation, but causes the rapid tyrosine phosphorylation of several intracellular proteins. Our results indicate that activation of intracellular tyrosine kinases is not secondary to Ca2+ mobilization and protein kinase C activation in lysophosphatidic acid-stimulated platelets.

Original languageEnglish
Pages (from-to)253-255
Number of pages3
JournalBlood Coagulation and Fibrinolysis
Volume7
Issue number2
Publication statusPublished - 1996

Fingerprint

Platelet Activation
Blood Platelets
Phosphorylation
Type C Phospholipases
GTP-Binding Proteins
Protein-Tyrosine Kinases
Protein Kinase C
Tyrosine
Phospholipids
Gels
lysophosphatidic acid
Membranes
Proteins

Keywords

  • Aggregation
  • G-proteins
  • Phospholipase C
  • Protein-tyrosine phosphorylation
  • Secretion

ASJC Scopus subject areas

  • Hematology

Cite this

Torti, M., Tolnai Festetics, E., Bertoni, A., Sinigaglia, F., & Balduini, C. (1996). Activation of human platelets by lysophosphatidic acid. Blood Coagulation and Fibrinolysis, 7(2), 253-255.

Activation of human platelets by lysophosphatidic acid. / Torti, M.; Tolnai Festetics, E.; Bertoni, A.; Sinigaglia, F.; Balduini, C.

In: Blood Coagulation and Fibrinolysis, Vol. 7, No. 2, 1996, p. 253-255.

Research output: Contribution to journalArticle

Torti, M, Tolnai Festetics, E, Bertoni, A, Sinigaglia, F & Balduini, C 1996, 'Activation of human platelets by lysophosphatidic acid', Blood Coagulation and Fibrinolysis, vol. 7, no. 2, pp. 253-255.
Torti M, Tolnai Festetics E, Bertoni A, Sinigaglia F, Balduini C. Activation of human platelets by lysophosphatidic acid. Blood Coagulation and Fibrinolysis. 1996;7(2):253-255.
Torti, M. ; Tolnai Festetics, E. ; Bertoni, A. ; Sinigaglia, F. ; Balduini, C. / Activation of human platelets by lysophosphatidic acid. In: Blood Coagulation and Fibrinolysis. 1996 ; Vol. 7, No. 2. pp. 253-255.
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